Neuroprotective effects of active ingredients isolated from Pegasus laternarius on cultured cerebral neurons

Mengtao Li, Minhui Chen, Hai Huang, Wucheng Tao, Jihong Cui, Hui Xiang

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Seamoth (Pegasus laternarius Cuvier) is extensively used to treat various diseases on the coastland of Guangdong Province in China, such as scrofula, cough, and diarrhea. The total extract of Pegasus laternarius (EP) was subjected to column chromatography to acquire three different constituents (EPC1, EPC2, and EPC3). Cerebral neuron injury was induced by glutamate, H 2O 2, and serum deprivation. After treating with or without different extracts, cell viability was assessed with the MTT (3-(4,5-Dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) assay, and cell apoptosis was analyzed with Hoechst 33258 staining and agarose gel electrophoresis. We also determined the levels of lactate dehydrogenase (LDH), maleic dialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px). The results showed that both EP and EPC2 promoted the outgrowth of cultural neurons, increased antioxidant enzyme activity, and protected neurons from neuronal injury or apoptosis induced by glutamate, H 2O 2, and serum deprivation. EPC1 and EPC3 had little or no effect on neurons. These results suggest that the active ingredients obtained from Pegasus laternarius have potential neuroprotective effects on injured neurons by promoting the outgrowth of cultured neurons, increasing the activity of intracellular antioxidants, and exerting antiapoptotic effects. This neuroprotection may be attributable to specific active ingredients, such as taurine, novel ceramide, and cholesterol.

Original languageEnglish (US)
Pages (from-to)73-82
Number of pages10
JournalCellular and Molecular Neurobiology
Volume31
Issue number1
DOIs
StatePublished - Jan 2011
Externally publishedYes

Keywords

  • Active ingredients
  • Apoptosis
  • Cerebral neurons
  • Neuroprotective effect
  • Pegasus laternarius
  • Taurine

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

Fingerprint Dive into the research topics of 'Neuroprotective effects of active ingredients isolated from Pegasus laternarius on cultured cerebral neurons'. Together they form a unique fingerprint.

Cite this