Neuroprotection of luteolin against methylmercury-induced toxicity in lobster cockroach Nauphoeta cinerea

Isaac A. Adedara, Denis B. Rosemberg, Diogo O. Souza, Ebenezer O. Farombi, Michael Aschner, Joao B T Rocha

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Luteolin (3', 4', 5, 7-tetrahydroxyflavone) is a polyphenolic compound found in foods of plant origin and has been reported to possess antioxidant and neuroprotective properties. However, there is dearth of information on the beneficial effects of luteolin on methylmercury (MeHg), a long-established neurotoxic compound in animals and humans. This study evaluated the effect of luteolin on MeHg-induced behavioral and biochemical deficits, using lobster cockroach Nauphoeta cinerea as an alternative and complementary animal model. The insects were exposed for 35 consecutive days to either MeHg alone (0.05 mg/g feed) or in combination with luteolin at 0.25, 0.5 and 1.0 mg/g feed. Locomotor behavior was assessed using video-tracking software during a 10-min trial in a novel arena and subsequently, biochemical analyses were carried out using the cockroaches' heads. Luteolin supplementation dose-dependently reversed the MeHg-induced locomotor deficits and enhanced the exploratory profiles of MeHg-exposed cockroaches as confirmed by track and occupancy plot analyses. Luteolin reversed the MeHg-induced acetylcholinesterase activity inhibition, decreased dichlorofluorescein oxidation and lipid peroxidation levels, but increased total thiol level and catalase and glutathione S-transferase activities in the treated cockroaches. In conclusion, luteolin prevented oxidative stress indices and neurobehavioral deficits in a Nauphoeta cinerea model of MeHg toxicity.

Original languageEnglish (US)
Pages (from-to)243-251
Number of pages9
JournalEnvironmental Toxicology and Pharmacology
Volume42
DOIs
StatePublished - Mar 1 2016

Fingerprint

Luteolin
Cockroaches
Toxicity
Animals
Polyphenolic compounds
Edible Plants
Oxidative stress
Neuroprotection
Acetylcholinesterase
Glutathione Transferase
Sulfhydryl Compounds
Catalase
Lipid Peroxidation
Insects
Oxidative Stress
Software
Animal Models
Antioxidants
Head
Lipids

Keywords

  • Alternative model
  • Luteolin
  • Methylmercury
  • Nauphoeta cinerea
  • Neurotoxicity

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis

Cite this

Neuroprotection of luteolin against methylmercury-induced toxicity in lobster cockroach Nauphoeta cinerea. / Adedara, Isaac A.; Rosemberg, Denis B.; Souza, Diogo O.; Farombi, Ebenezer O.; Aschner, Michael; Rocha, Joao B T.

In: Environmental Toxicology and Pharmacology, Vol. 42, 01.03.2016, p. 243-251.

Research output: Contribution to journalArticle

Adedara, Isaac A. ; Rosemberg, Denis B. ; Souza, Diogo O. ; Farombi, Ebenezer O. ; Aschner, Michael ; Rocha, Joao B T. / Neuroprotection of luteolin against methylmercury-induced toxicity in lobster cockroach Nauphoeta cinerea. In: Environmental Toxicology and Pharmacology. 2016 ; Vol. 42. pp. 243-251.
@article{b6fbff180901443baf5e5183ee0dee9c,
title = "Neuroprotection of luteolin against methylmercury-induced toxicity in lobster cockroach Nauphoeta cinerea",
abstract = "Luteolin (3', 4', 5, 7-tetrahydroxyflavone) is a polyphenolic compound found in foods of plant origin and has been reported to possess antioxidant and neuroprotective properties. However, there is dearth of information on the beneficial effects of luteolin on methylmercury (MeHg), a long-established neurotoxic compound in animals and humans. This study evaluated the effect of luteolin on MeHg-induced behavioral and biochemical deficits, using lobster cockroach Nauphoeta cinerea as an alternative and complementary animal model. The insects were exposed for 35 consecutive days to either MeHg alone (0.05 mg/g feed) or in combination with luteolin at 0.25, 0.5 and 1.0 mg/g feed. Locomotor behavior was assessed using video-tracking software during a 10-min trial in a novel arena and subsequently, biochemical analyses were carried out using the cockroaches' heads. Luteolin supplementation dose-dependently reversed the MeHg-induced locomotor deficits and enhanced the exploratory profiles of MeHg-exposed cockroaches as confirmed by track and occupancy plot analyses. Luteolin reversed the MeHg-induced acetylcholinesterase activity inhibition, decreased dichlorofluorescein oxidation and lipid peroxidation levels, but increased total thiol level and catalase and glutathione S-transferase activities in the treated cockroaches. In conclusion, luteolin prevented oxidative stress indices and neurobehavioral deficits in a Nauphoeta cinerea model of MeHg toxicity.",
keywords = "Alternative model, Luteolin, Methylmercury, Nauphoeta cinerea, Neurotoxicity",
author = "Adedara, {Isaac A.} and Rosemberg, {Denis B.} and Souza, {Diogo O.} and Farombi, {Ebenezer O.} and Michael Aschner and Rocha, {Joao B T}",
year = "2016",
month = "3",
day = "1",
doi = "10.1016/j.etap.2016.02.001",
language = "English (US)",
volume = "42",
pages = "243--251",
journal = "Environmental Toxicology and Pharmacology",
issn = "1382-6689",
publisher = "Elsevier",

}

TY - JOUR

T1 - Neuroprotection of luteolin against methylmercury-induced toxicity in lobster cockroach Nauphoeta cinerea

AU - Adedara, Isaac A.

AU - Rosemberg, Denis B.

AU - Souza, Diogo O.

AU - Farombi, Ebenezer O.

AU - Aschner, Michael

AU - Rocha, Joao B T

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Luteolin (3', 4', 5, 7-tetrahydroxyflavone) is a polyphenolic compound found in foods of plant origin and has been reported to possess antioxidant and neuroprotective properties. However, there is dearth of information on the beneficial effects of luteolin on methylmercury (MeHg), a long-established neurotoxic compound in animals and humans. This study evaluated the effect of luteolin on MeHg-induced behavioral and biochemical deficits, using lobster cockroach Nauphoeta cinerea as an alternative and complementary animal model. The insects were exposed for 35 consecutive days to either MeHg alone (0.05 mg/g feed) or in combination with luteolin at 0.25, 0.5 and 1.0 mg/g feed. Locomotor behavior was assessed using video-tracking software during a 10-min trial in a novel arena and subsequently, biochemical analyses were carried out using the cockroaches' heads. Luteolin supplementation dose-dependently reversed the MeHg-induced locomotor deficits and enhanced the exploratory profiles of MeHg-exposed cockroaches as confirmed by track and occupancy plot analyses. Luteolin reversed the MeHg-induced acetylcholinesterase activity inhibition, decreased dichlorofluorescein oxidation and lipid peroxidation levels, but increased total thiol level and catalase and glutathione S-transferase activities in the treated cockroaches. In conclusion, luteolin prevented oxidative stress indices and neurobehavioral deficits in a Nauphoeta cinerea model of MeHg toxicity.

AB - Luteolin (3', 4', 5, 7-tetrahydroxyflavone) is a polyphenolic compound found in foods of plant origin and has been reported to possess antioxidant and neuroprotective properties. However, there is dearth of information on the beneficial effects of luteolin on methylmercury (MeHg), a long-established neurotoxic compound in animals and humans. This study evaluated the effect of luteolin on MeHg-induced behavioral and biochemical deficits, using lobster cockroach Nauphoeta cinerea as an alternative and complementary animal model. The insects were exposed for 35 consecutive days to either MeHg alone (0.05 mg/g feed) or in combination with luteolin at 0.25, 0.5 and 1.0 mg/g feed. Locomotor behavior was assessed using video-tracking software during a 10-min trial in a novel arena and subsequently, biochemical analyses were carried out using the cockroaches' heads. Luteolin supplementation dose-dependently reversed the MeHg-induced locomotor deficits and enhanced the exploratory profiles of MeHg-exposed cockroaches as confirmed by track and occupancy plot analyses. Luteolin reversed the MeHg-induced acetylcholinesterase activity inhibition, decreased dichlorofluorescein oxidation and lipid peroxidation levels, but increased total thiol level and catalase and glutathione S-transferase activities in the treated cockroaches. In conclusion, luteolin prevented oxidative stress indices and neurobehavioral deficits in a Nauphoeta cinerea model of MeHg toxicity.

KW - Alternative model

KW - Luteolin

KW - Methylmercury

KW - Nauphoeta cinerea

KW - Neurotoxicity

UR - http://www.scopus.com/inward/record.url?scp=84984565473&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84984565473&partnerID=8YFLogxK

U2 - 10.1016/j.etap.2016.02.001

DO - 10.1016/j.etap.2016.02.001

M3 - Article

VL - 42

SP - 243

EP - 251

JO - Environmental Toxicology and Pharmacology

JF - Environmental Toxicology and Pharmacology

SN - 1382-6689

ER -