Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary

Lloyd D. Fricker, Alexandre K. Tashima, Amanda K. Fakira, Ute Hochgeschwender, William C. Wetsel, Lakshmi A. Devi

Research output: Contribution to journalArticlepeer-review

Abstract

We report a method to identify neuropeptides in specific cell types, using mice with a conditional deletion of carboxypeptidase E. It was validated by deleting CPE in proopiomelanocortin cells. Surprisingly, these mice have greatly reduced levels of the anorexigenic peptide α-MSH but are not obese.

Original languageEnglish (US)
Pages (from-to)105-112.e4
JournalCell Chemical Biology
Volume28
Issue number1
DOIs
StatePublished - Jan 21 2021

Keywords

  • Proteomics
  • carboxypeptidase
  • neuropeptide
  • neuropeptidomics
  • peptidomics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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