TY - JOUR
T1 - Neuropeptide Y overexpression in the preweanling Zucker (fa/fa) rat
AU - Kowalski, Timothy J.
AU - Houpt, Thomas A.
AU - Jahng, Jeongwon
AU - Okada, Nori
AU - Liu, Shun Mei
AU - Chua, Streamson C.
AU - Smith, Gerard P.
N1 - Funding Information:
This work was funded by NIH Grants MH 00149, T32 MH 18390, and MH 15455. We thank Drs. Aron Weller and James Gibbs for constructive criticism of this article.
PY - 1999/10
Y1 - 1999/10
N2 - Hypothalamic preproNPY overexpression in the Zucker fatty (fa/fa) rat was examined. In situ hybridization was used to determine the relative level of preproNPY mRNA in the arcuate nucleus of +/+, +/fa, and fa/fa pups aged postnatal day 2 (P2), 5, 9, 12, or 25. The relative optical density (ROD) of probe hybridization in the arcuate, the area of hybridization (A), and the product of ROD x A (a measure of total arcuate preproNPY mRNA hybridization) were measured. Values were normalized to the mean +/fa value within each litter. Initial analysis showed that preproNPY mRNA hybridization (ROD x A) in fa/fa pups was significantly higher than +/fa and +/+ pups on P9, 12, and 25, and significantly higher than +/fa on P5. No significant difference between lean (+/+ and +/fa) genotypes, however, were observed at any age tested. Values from the lean genotypes were, therefore, pooled, and data were normalized to the mean value of lean animals for analysis. This analysis revealed that preproNPY mRNA hybridization in fa/fa pups was higher than lean littermates as early as P2. Copyright (C) 1999 Elsevier Science Inc.
AB - Hypothalamic preproNPY overexpression in the Zucker fatty (fa/fa) rat was examined. In situ hybridization was used to determine the relative level of preproNPY mRNA in the arcuate nucleus of +/+, +/fa, and fa/fa pups aged postnatal day 2 (P2), 5, 9, 12, or 25. The relative optical density (ROD) of probe hybridization in the arcuate, the area of hybridization (A), and the product of ROD x A (a measure of total arcuate preproNPY mRNA hybridization) were measured. Values were normalized to the mean +/fa value within each litter. Initial analysis showed that preproNPY mRNA hybridization (ROD x A) in fa/fa pups was significantly higher than +/fa and +/+ pups on P9, 12, and 25, and significantly higher than +/fa on P5. No significant difference between lean (+/+ and +/fa) genotypes, however, were observed at any age tested. Values from the lean genotypes were, therefore, pooled, and data were normalized to the mean value of lean animals for analysis. This analysis revealed that preproNPY mRNA hybridization in fa/fa pups was higher than lean littermates as early as P2. Copyright (C) 1999 Elsevier Science Inc.
KW - Arcuate nucleus
KW - Development of obesity
KW - Genetic obesity
KW - Hypothalamus
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U2 - 10.1016/S0031-9384(99)00095-5
DO - 10.1016/S0031-9384(99)00095-5
M3 - Article
C2 - 10549888
AN - SCOPUS:0032879096
SN - 0031-9384
VL - 67
SP - 521
EP - 525
JO - Physiology and Behavior
JF - Physiology and Behavior
IS - 4
ER -
