Neuronal oxidative injury and biomarkers of lipid peroxidation

Dejan Milatovic, Michael Aschner

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Oxidative stress is implicated as one of the major underlying mechanisms in a variety of human diseases. Reactive radicals, derived primarily from molecular oxygen, readily attack a variety of critical biological molecules, including DNA, cellular proteins, and lipids. Since lipid peroxidation is a central feature of cerebral oxidant injury, measurements of peroxidation products, such as F2-isoprostanes (F2-IsoPs) and F 4-neuroprostanes (F4-NeuroPs), represent the most accurate approaches to identify oxidative injury in vivo. Our laboratory uses a gas chromatography/mass spectrometry negative ion chemical ionization with select ion monitoring for quantification of these biomarkers of oxidative stress in a plethora of biological media. This review summarizes state-of-the-art methodology for F2-IsoPs and F4-NeuroPs analyses and discusses the utility of these prostaglandin-like compounds as in vivo biomarkers of neuronal oxidative injury.

Original languageEnglish (US)
Title of host publicationCell Culture Techniques
EditorsMichael Aschner, Cristina Sunol, Anna Bal-Price
Pages349-363
Number of pages15
DOIs
StatePublished - Mar 18 2011

Publication series

NameNeuromethods
Volume56
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

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Keywords

  • F-isoprostanes
  • F-neuroprostanes
  • Lipid peroxidation
  • Neuronal tissue
  • Oxidative damage

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

Cite this

Milatovic, D., & Aschner, M. (2011). Neuronal oxidative injury and biomarkers of lipid peroxidation. In M. Aschner, C. Sunol, & A. Bal-Price (Eds.), Cell Culture Techniques (pp. 349-363). (Neuromethods; Vol. 56). https://doi.org/10.1007/978-1-61779-077-5_17