Neuronal-like differentiation of single versus multiple treatments with human amnion-derived mesenchymal stem cells induced by basic fibroblast growth factor

Hongliang Jiao, Fangxia Guan, Xiang Hu, Jianbin Li, Hong Shan, Wei Li, Jun Li, Ying Du, Bo Yang, Yunfan Zhou

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Cultures from multiple portions of umbilical cord blood mesenchymal stem cells have been shown to undergo more rapid proliferation and attachment than single portions. OBJECTIVE: To observe growth of basic fibroblast growth factor (bFGF)-induced cultures of human amnion-derived mesenchymal stem cells (AMSCs) and differentiation into neuronal-like cells. DESIGN, TIME AND SETTING: Comparative observation. The study was performed at the Laboratory of Microbiology and Immunology, Basic Medical School of Zhengzhou University from January to May 2008. METHODS: Amnia from full-term, uterine-incision delivery were donated by 12 healthy women. AMSCs were obtained by cell separation and culture techniques, and were passaged and induced by bFGF. From the third passage, a total of 1 mL AMSCs, at a density of 1.0 × 104/mL, was separately harvested from six samples, which served as group A. A total of 1 mL AMSCs, at a density of 1.0 × 104/mL, was harvested separately from the remaining six samples, which served as group B. A total of 0.5 mL from the six samples of group A and 0.5 mL from the six samples of group B were combined to form group C. MAIN OUTCOME MEASURES: Differences in cell quantity among the three groups were compared by cell quantification and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis. Expression of a glial cell marker, neuron-specific enolase, and nestin was detected in the three groups by immunocytochemistry. RESULTS: Cell quantification and MTT analysis of live cells, as well as AMSC absorbance, were significantly greater in group C compared with groups A and B at 18 days of culture (P < 0.05), and no significant difference was observed between groups A and B. Glial fibrillary acidic protein, neuron-specific enolase, and nestin were expressed in all groups following bFGF induction. CONCLUSION: Mixed AMSC cultures promoted proliferation, and bFGF-induced AMSCs differentiated into neuronal-like cells.

Original languageEnglish (US)
Pages (from-to)694-698
Number of pages5
JournalNeural Regeneration Research
Volume4
Issue number9
DOIs
StatePublished - Sep 2009
Externally publishedYes

Fingerprint

Amnion
Fibroblast Growth Factor 2
Mesenchymal Stromal Cells
Nestin
Phosphopyruvate Hydratase
Therapeutics
Cell Culture Techniques
Cell Separation
Glial Fibrillary Acidic Protein
Microbiology
Allergy and Immunology
Medical Schools
Fetal Blood
Neuroglia
Cell Differentiation
Immunohistochemistry
Observation
Growth

Keywords

  • Amnion
  • Induction
  • Mesenchymal stem cells
  • Mixed culture

ASJC Scopus subject areas

  • Developmental Neuroscience

Cite this

Neuronal-like differentiation of single versus multiple treatments with human amnion-derived mesenchymal stem cells induced by basic fibroblast growth factor. / Jiao, Hongliang; Guan, Fangxia; Hu, Xiang; Li, Jianbin; Shan, Hong; Li, Wei; Li, Jun; Du, Ying; Yang, Bo; Zhou, Yunfan.

In: Neural Regeneration Research, Vol. 4, No. 9, 09.2009, p. 694-698.

Research output: Contribution to journalArticle

Jiao, Hongliang ; Guan, Fangxia ; Hu, Xiang ; Li, Jianbin ; Shan, Hong ; Li, Wei ; Li, Jun ; Du, Ying ; Yang, Bo ; Zhou, Yunfan. / Neuronal-like differentiation of single versus multiple treatments with human amnion-derived mesenchymal stem cells induced by basic fibroblast growth factor. In: Neural Regeneration Research. 2009 ; Vol. 4, No. 9. pp. 694-698.
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T1 - Neuronal-like differentiation of single versus multiple treatments with human amnion-derived mesenchymal stem cells induced by basic fibroblast growth factor

AU - Jiao, Hongliang

AU - Guan, Fangxia

AU - Hu, Xiang

AU - Li, Jianbin

AU - Shan, Hong

AU - Li, Wei

AU - Li, Jun

AU - Du, Ying

AU - Yang, Bo

AU - Zhou, Yunfan

PY - 2009/9

Y1 - 2009/9

N2 - BACKGROUND: Cultures from multiple portions of umbilical cord blood mesenchymal stem cells have been shown to undergo more rapid proliferation and attachment than single portions. OBJECTIVE: To observe growth of basic fibroblast growth factor (bFGF)-induced cultures of human amnion-derived mesenchymal stem cells (AMSCs) and differentiation into neuronal-like cells. DESIGN, TIME AND SETTING: Comparative observation. The study was performed at the Laboratory of Microbiology and Immunology, Basic Medical School of Zhengzhou University from January to May 2008. METHODS: Amnia from full-term, uterine-incision delivery were donated by 12 healthy women. AMSCs were obtained by cell separation and culture techniques, and were passaged and induced by bFGF. From the third passage, a total of 1 mL AMSCs, at a density of 1.0 × 104/mL, was separately harvested from six samples, which served as group A. A total of 1 mL AMSCs, at a density of 1.0 × 104/mL, was harvested separately from the remaining six samples, which served as group B. A total of 0.5 mL from the six samples of group A and 0.5 mL from the six samples of group B were combined to form group C. MAIN OUTCOME MEASURES: Differences in cell quantity among the three groups were compared by cell quantification and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis. Expression of a glial cell marker, neuron-specific enolase, and nestin was detected in the three groups by immunocytochemistry. RESULTS: Cell quantification and MTT analysis of live cells, as well as AMSC absorbance, were significantly greater in group C compared with groups A and B at 18 days of culture (P < 0.05), and no significant difference was observed between groups A and B. Glial fibrillary acidic protein, neuron-specific enolase, and nestin were expressed in all groups following bFGF induction. CONCLUSION: Mixed AMSC cultures promoted proliferation, and bFGF-induced AMSCs differentiated into neuronal-like cells.

AB - BACKGROUND: Cultures from multiple portions of umbilical cord blood mesenchymal stem cells have been shown to undergo more rapid proliferation and attachment than single portions. OBJECTIVE: To observe growth of basic fibroblast growth factor (bFGF)-induced cultures of human amnion-derived mesenchymal stem cells (AMSCs) and differentiation into neuronal-like cells. DESIGN, TIME AND SETTING: Comparative observation. The study was performed at the Laboratory of Microbiology and Immunology, Basic Medical School of Zhengzhou University from January to May 2008. METHODS: Amnia from full-term, uterine-incision delivery were donated by 12 healthy women. AMSCs were obtained by cell separation and culture techniques, and were passaged and induced by bFGF. From the third passage, a total of 1 mL AMSCs, at a density of 1.0 × 104/mL, was separately harvested from six samples, which served as group A. A total of 1 mL AMSCs, at a density of 1.0 × 104/mL, was harvested separately from the remaining six samples, which served as group B. A total of 0.5 mL from the six samples of group A and 0.5 mL from the six samples of group B were combined to form group C. MAIN OUTCOME MEASURES: Differences in cell quantity among the three groups were compared by cell quantification and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis. Expression of a glial cell marker, neuron-specific enolase, and nestin was detected in the three groups by immunocytochemistry. RESULTS: Cell quantification and MTT analysis of live cells, as well as AMSC absorbance, were significantly greater in group C compared with groups A and B at 18 days of culture (P < 0.05), and no significant difference was observed between groups A and B. Glial fibrillary acidic protein, neuron-specific enolase, and nestin were expressed in all groups following bFGF induction. CONCLUSION: Mixed AMSC cultures promoted proliferation, and bFGF-induced AMSCs differentiated into neuronal-like cells.

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