Neuron to glia signaling triggers myelin membrane exocytosis from endosomal storage sites

Katarina Trajkovic, Ajit Singh Dhaunchak, J. Tiago Goncalves, Dirk Wenzel, Anja Schneider, Gertrude Bunt, Klaus Armin Nave, Mikael Simons

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

During vertebrate brain development, axons are enwrapped by myelin, an insulating membrane produced by oligodendrocytes. Neuron-derived signaling molecules are temporally and spatially required to coordinate oligodendrocyte differentiation. In this study, we show that neurons regulate myelin membrane traf. cking in oligodendrocytes. In the absence of neurons, the major myelin membrane protein, the proteolipid protein (PLP), is internalized and stored in late endosomes/lysosomes (LEs/Ls) by a cholesterol-dependent and clathrinindependent endocytosis pathway that requires actin and the RhoA guanosine triphosphatase. Upon maturation, the rate of endocytosis is reduced, and a cAMP-dependent neuronal signal triggers the transport of PLP from LEs/Ls to the plasma membrane. These . ndings reveal a fundamental and novel role of LEs/Ls in oligodendrocytes: to store and release PLP in a regulated fashion. The release of myelin membrane from LEs/Ls by neuronal signals may represent a mechanism to control myelin membrane growth.

Original languageEnglish (US)
Pages (from-to)937-948
Number of pages12
JournalJournal of Cell Biology
Volume172
Issue number6
DOIs
StatePublished - Mar 13 2006
Externally publishedYes

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Endosomes
Oligodendroglia
Exocytosis
Myelin Sheath
Lysosomes
Proteolipids
Neuroglia
Neurons
Membranes
Endocytosis
Myelin Proteins
Guanosine
Axons
Vertebrates
Actins
Carrier Proteins
Membrane Proteins
Proteins
Cholesterol
Cell Membrane

ASJC Scopus subject areas

  • Cell Biology

Cite this

Trajkovic, K., Dhaunchak, A. S., Goncalves, J. T., Wenzel, D., Schneider, A., Bunt, G., ... Simons, M. (2006). Neuron to glia signaling triggers myelin membrane exocytosis from endosomal storage sites. Journal of Cell Biology, 172(6), 937-948. https://doi.org/10.1083/jcb.200509022

Neuron to glia signaling triggers myelin membrane exocytosis from endosomal storage sites. / Trajkovic, Katarina; Dhaunchak, Ajit Singh; Goncalves, J. Tiago; Wenzel, Dirk; Schneider, Anja; Bunt, Gertrude; Nave, Klaus Armin; Simons, Mikael.

In: Journal of Cell Biology, Vol. 172, No. 6, 13.03.2006, p. 937-948.

Research output: Contribution to journalArticle

Trajkovic, K, Dhaunchak, AS, Goncalves, JT, Wenzel, D, Schneider, A, Bunt, G, Nave, KA & Simons, M 2006, 'Neuron to glia signaling triggers myelin membrane exocytosis from endosomal storage sites', Journal of Cell Biology, vol. 172, no. 6, pp. 937-948. https://doi.org/10.1083/jcb.200509022
Trajkovic, Katarina ; Dhaunchak, Ajit Singh ; Goncalves, J. Tiago ; Wenzel, Dirk ; Schneider, Anja ; Bunt, Gertrude ; Nave, Klaus Armin ; Simons, Mikael. / Neuron to glia signaling triggers myelin membrane exocytosis from endosomal storage sites. In: Journal of Cell Biology. 2006 ; Vol. 172, No. 6. pp. 937-948.
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