TY - JOUR
T1 - Neuron to glia signaling triggers myelin membrane exocytosis from endosomal storage sites
AU - Trajkovic, Katarina
AU - Dhaunchak, Ajit Singh
AU - Goncalves, José T.
AU - Wenzel, Dirk
AU - Schneider, Anja
AU - Bunt, Gertrude
AU - Nave, Klaus Armin
AU - Simons, Mikael
PY - 2006/3/13
Y1 - 2006/3/13
N2 - During vertebrate brain development, axons are enwrapped by myelin, an insulating membrane produced by oligodendrocytes. Neuron-derived signaling molecules are temporally and spatially required to coordinate oligodendrocyte differentiation. In this study, we show that neurons regulate myelin membrane traf. cking in oligodendrocytes. In the absence of neurons, the major myelin membrane protein, the proteolipid protein (PLP), is internalized and stored in late endosomes/lysosomes (LEs/Ls) by a cholesterol-dependent and clathrinindependent endocytosis pathway that requires actin and the RhoA guanosine triphosphatase. Upon maturation, the rate of endocytosis is reduced, and a cAMP-dependent neuronal signal triggers the transport of PLP from LEs/Ls to the plasma membrane. These . ndings reveal a fundamental and novel role of LEs/Ls in oligodendrocytes: to store and release PLP in a regulated fashion. The release of myelin membrane from LEs/Ls by neuronal signals may represent a mechanism to control myelin membrane growth.
AB - During vertebrate brain development, axons are enwrapped by myelin, an insulating membrane produced by oligodendrocytes. Neuron-derived signaling molecules are temporally and spatially required to coordinate oligodendrocyte differentiation. In this study, we show that neurons regulate myelin membrane traf. cking in oligodendrocytes. In the absence of neurons, the major myelin membrane protein, the proteolipid protein (PLP), is internalized and stored in late endosomes/lysosomes (LEs/Ls) by a cholesterol-dependent and clathrinindependent endocytosis pathway that requires actin and the RhoA guanosine triphosphatase. Upon maturation, the rate of endocytosis is reduced, and a cAMP-dependent neuronal signal triggers the transport of PLP from LEs/Ls to the plasma membrane. These . ndings reveal a fundamental and novel role of LEs/Ls in oligodendrocytes: to store and release PLP in a regulated fashion. The release of myelin membrane from LEs/Ls by neuronal signals may represent a mechanism to control myelin membrane growth.
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U2 - 10.1083/jcb.200509022
DO - 10.1083/jcb.200509022
M3 - Article
C2 - 16520383
AN - SCOPUS:33644916118
SN - 0021-9525
VL - 172
SP - 937
EP - 948
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 6
ER -