Neurobehavioral and biochemical changes in Nauphoeta cinerea following dietary exposure to chlorpyrifos

Isaac A. Adedara, Denis B. Rosemberg, Diego de Souza, Ebenezer O. Farombi, Michael Aschner, Diogo O. Souza, Joao B T Rocha

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The present study aimed to increase our understanding about the mode of toxic action of organophosphate pesticides in insects by evaluating the biochemical and neurobehavioral characteristics in Nauphoeta cinerea exposed to chlorpyrifos (CPF)-contaminated diet. The insects were exposed for 35 consecutive days to CPF at 0.078, 0.15625, 0.3125 and 0.625 μg/g feed. Locomotor behavior was assessed for a 10-min trial in a novel arena and subsequently, biochemical analyses were carried out using the cockroaches' heads. In comparison to control, CPF-exposed cockroaches showed significant decreases in the total distance traveled, body rotation, turn angle and meandering, along with significant increase in the number of falls, time and episodes of immobility. The marked decrease in the exploratory profiles of CPF-exposed cockroaches was confirmed by track plots, whereas occupancy plot analyses showed a progressive dispersion at 0.15625 μg/g feed group. Moreover, the heads of CPF-exposed cockroaches showed marked decrease in acetylcholinesterase activity and antioxidant status with concomitant significant elevation in dichlorofluorescein oxidation and lipid peroxidation levels in CPF-treated cockroaches. Gas Chromatography-Mass Spectrometry analyses revealed bioaccumulation of CPF in cockroaches exposed to concentrations above 0.078 μg/g feed. The findings from this investigation showed N. cinerea as a value model organism for the risk assessment of environmental organophosphate contamination in insects.

Original languageEnglish (US)
Pages (from-to)22-30
Number of pages9
JournalPesticide Biochemistry and Physiology
Volume130
DOIs
StatePublished - Jun 1 2016

Fingerprint

Nauphoeta cinerea
Chlorpyrifos
dietary exposure
chlorpyrifos
Cockroaches
Blattodea
Insects
Organophosphates
insects
Toxic Actions
organophosphorus pesticides
environmental assessment
organophosphorus compounds
bioaccumulation
Acetylcholinesterase
acetylcholinesterase
Pesticides
Gas Chromatography-Mass Spectrometry
Lipid Peroxidation
lipid peroxidation

Keywords

  • Chlorpyrifos
  • Environmental contaminants
  • Nauphoeta cinerea
  • Neurobehavior
  • Oxidative stress

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Health, Toxicology and Mutagenesis

Cite this

Neurobehavioral and biochemical changes in Nauphoeta cinerea following dietary exposure to chlorpyrifos. / Adedara, Isaac A.; Rosemberg, Denis B.; de Souza, Diego; Farombi, Ebenezer O.; Aschner, Michael; Souza, Diogo O.; Rocha, Joao B T.

In: Pesticide Biochemistry and Physiology, Vol. 130, 01.06.2016, p. 22-30.

Research output: Contribution to journalArticle

Adedara, Isaac A. ; Rosemberg, Denis B. ; de Souza, Diego ; Farombi, Ebenezer O. ; Aschner, Michael ; Souza, Diogo O. ; Rocha, Joao B T. / Neurobehavioral and biochemical changes in Nauphoeta cinerea following dietary exposure to chlorpyrifos. In: Pesticide Biochemistry and Physiology. 2016 ; Vol. 130. pp. 22-30.
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