Neuregulin and erbB receptor expression in normal and diseased human white matter

Barbara Cannella, David Pitt, Mark Marchionni, Cedric S. Raine

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Human white matter from non-neurologic cases, multiple sclerosis (MS) and other neurologic diseases (OND, inflammatory and non-inflammatory), was subjected to immunocytochemistry and Western blotting for expression of the neuregulin, glial growth factor-2 (GGF2), and its receptors, erbB2, erbB3 and erbB4. GGF2 has previously been shown to have mitogenic effects upon oligodendrocytes in vitro and an enhancing effect upon remyelination in animals with autoimmune demyelination. In all types of human white matter examined, expression of the ligand GGF2 and its three receptors was consistently found on oligodendrocytes, with higher levels being seen in cases of MS. Expression was also seen, albeit at lower levels, on astrocytes and microglial cells, the latter most commonly in MS and OND. In human lymph node tissue, some lymphocytes were positive for erbB2, erbB3 and erbB4. Western blots confirmed the presence of all three receptors in normal, MS and OND white matter. GGF2 and erbB receptor expression did not correlate with areas of remyelination and reactivity occurred throughout the tissue, with some increase in intensity at the edge of MS lesions. Examination of precursor oligodendrocyte immunoreactivity (with anti-PDGF-Rα and NG2), revealed widespread expression throughout both normal and diseased white matter. The presence of GGF2 and its receptors on oligodendrocytes and lymphocytes render this cell type a candidate for functional signaling via this pathway, perhaps in relationship to myelinating activity. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)233-242
Number of pages10
JournalJournal of Neuroimmunology
Volume100
Issue number1-2
DOIs
StatePublished - Dec 1999

Fingerprint

Neuregulins
Neuregulin-1
Multiple Sclerosis
Oligodendroglia
Western Blotting
Lymphocytes
Growth Factor Receptors
Demyelinating Diseases
Nervous System Diseases
Astrocytes
Lymph Nodes
Immunohistochemistry
ErbB Receptors
White Matter
Ligands

Keywords

  • erbB receptor
  • Human white matter
  • Immunocytochemistry
  • Microglia
  • Multiple sclerosis
  • Neuregulin
  • Oligodendrocyte
  • Western blotting

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

Neuregulin and erbB receptor expression in normal and diseased human white matter. / Cannella, Barbara; Pitt, David; Marchionni, Mark; Raine, Cedric S.

In: Journal of Neuroimmunology, Vol. 100, No. 1-2, 12.1999, p. 233-242.

Research output: Contribution to journalArticle

Cannella, Barbara ; Pitt, David ; Marchionni, Mark ; Raine, Cedric S. / Neuregulin and erbB receptor expression in normal and diseased human white matter. In: Journal of Neuroimmunology. 1999 ; Vol. 100, No. 1-2. pp. 233-242.
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