TY - JOUR
T1 - Neuregulin and erbB receptor expression in normal and diseased human white matter
AU - Cannella, Barbara
AU - Pitt, David
AU - Marchionni, Mark
AU - Raine, Cedric S.
N1 - Funding Information:
We thank Dr. Elimor Brand-Schieber, Gary Lelonek and Nina Langsam for essential assistance; Everett Swanson and Miriam Pakinghan for technical expertise; Drs. M. Greene (University of Pennsylvania); N. Latov (Columbia University), W. Stallcup (LaJolla, CA), C.-H. Heldin (Uppsala, Sweden) and A. Gard (University of S. Alabama), for kindly providing antibodies; and Patricia Cobban-Bond for preparation of the manuscript. Supported in part by USPHS grants NS 11920, NS 08952 and NS 07098; National Multiple Sclerosis Society Grant RG-1001-I-9; the Wollowick Family Foundation; and the Sol Goldman Charitable Trust.
Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1999/12
Y1 - 1999/12
N2 - Human white matter from non-neurologic cases, multiple sclerosis (MS) and other neurologic diseases (OND, inflammatory and non-inflammatory), was subjected to immunocytochemistry and Western blotting for expression of the neuregulin, glial growth factor-2 (GGF2), and its receptors, erbB2, erbB3 and erbB4. GGF2 has previously been shown to have mitogenic effects upon oligodendrocytes in vitro and an enhancing effect upon remyelination in animals with autoimmune demyelination. In all types of human white matter examined, expression of the ligand GGF2 and its three receptors was consistently found on oligodendrocytes, with higher levels being seen in cases of MS. Expression was also seen, albeit at lower levels, on astrocytes and microglial cells, the latter most commonly in MS and OND. In human lymph node tissue, some lymphocytes were positive for erbB2, erbB3 and erbB4. Western blots confirmed the presence of all three receptors in normal, MS and OND white matter. GGF2 and erbB receptor expression did not correlate with areas of remyelination and reactivity occurred throughout the tissue, with some increase in intensity at the edge of MS lesions. Examination of precursor oligodendrocyte immunoreactivity (with anti-PDGF-Rα and NG2), revealed widespread expression throughout both normal and diseased white matter. The presence of GGF2 and its receptors on oligodendrocytes and lymphocytes render this cell type a candidate for functional signaling via this pathway, perhaps in relationship to myelinating activity. Copyright (C) 1999 Elsevier Science B.V.
AB - Human white matter from non-neurologic cases, multiple sclerosis (MS) and other neurologic diseases (OND, inflammatory and non-inflammatory), was subjected to immunocytochemistry and Western blotting for expression of the neuregulin, glial growth factor-2 (GGF2), and its receptors, erbB2, erbB3 and erbB4. GGF2 has previously been shown to have mitogenic effects upon oligodendrocytes in vitro and an enhancing effect upon remyelination in animals with autoimmune demyelination. In all types of human white matter examined, expression of the ligand GGF2 and its three receptors was consistently found on oligodendrocytes, with higher levels being seen in cases of MS. Expression was also seen, albeit at lower levels, on astrocytes and microglial cells, the latter most commonly in MS and OND. In human lymph node tissue, some lymphocytes were positive for erbB2, erbB3 and erbB4. Western blots confirmed the presence of all three receptors in normal, MS and OND white matter. GGF2 and erbB receptor expression did not correlate with areas of remyelination and reactivity occurred throughout the tissue, with some increase in intensity at the edge of MS lesions. Examination of precursor oligodendrocyte immunoreactivity (with anti-PDGF-Rα and NG2), revealed widespread expression throughout both normal and diseased white matter. The presence of GGF2 and its receptors on oligodendrocytes and lymphocytes render this cell type a candidate for functional signaling via this pathway, perhaps in relationship to myelinating activity. Copyright (C) 1999 Elsevier Science B.V.
KW - Human white matter
KW - Immunocytochemistry
KW - Microglia
KW - Multiple sclerosis
KW - Neuregulin
KW - Oligodendrocyte
KW - Western blotting
KW - erbB receptor
UR - http://www.scopus.com/inward/record.url?scp=0033398455&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033398455&partnerID=8YFLogxK
U2 - 10.1016/S0165-5728(99)00201-5
DO - 10.1016/S0165-5728(99)00201-5
M3 - Article
C2 - 10695733
AN - SCOPUS:0033398455
SN - 0165-5728
VL - 100
SP - 233
EP - 242
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1-2
ER -