@article{faddbf96c70b4e78ae025f6d26bc1446,
title = "Nestin+NG2+ Cells Form a Reserve Stem Cell Population in the Mouse Prostate",
abstract = "In the prostate, stem and progenitor cell regenerative capacities have been ascribed to both basal and luminal epithelial cells. Here, we show that a rare subset of mesenchymal cells in the prostate are epithelial-primed Nestin-expressing cells (EPNECs) that can generate self-renewing prostate organoids with bipotential capacity. Upon transplantation, these EPNECs can form prostate gland tissue grafts at the clonal level. Lineage-tracing analyses show that cells marked by Nestin or NG2 transgenic mice contribute to prostate epithelium during organogenesis. In the adult, modest contributions in repeated rounds of regression and regeneration are observed, whereas prostate epithelial cells derived from Nestin/NG2-marked cells are dramatically increased after severe irradiation-induced organ damage. These results indicate that Nestin/NG2 expression marks a novel radioresistant prostate stem cell that is active during development and displays reserve stem cell activity for tissue maintenance.",
keywords = "Nestin, mesenchymal-to-epithelial transition, prostate stem cell",
author = "Maher Hanoun and Anna Arnal-Estap{\'e} and Maria Maryanovich and Zahalka, {Ali H.} and Bergren, {Sarah K.} and Chua, {Chee W.} and Avigdor Leftin and Brodin, {Patrik N.} and Shen, {Michael M.} and Chandan Guha and Frenette, {Paul S.}",
note = "Funding Information: We thank Colette Prophete, Lauren Schiff, and Paul Ciero for technical assistance; the Stem Cell FACS Facility and Einstein Flow Cytometry Core Facility for expert cell sorting; Dr. Toshihide Mizoguchi for advice; and Dr. Wenjun Guo for helpful scientific discussions. This work was supported by R01 grants from the National Institutes of Health (DK056638, DK112976, and HL069438 to P.S.F; DK076602 to M.M.S.), New York State Department of Health on Prostate Cancer Hypothesis Development Research (C31043GG) and NYSTEM (C029570). M.H. was supported by a fellowship of the German Research Foundation (DFG, Ha 6731/1-1). M.M. is a New York Stem Cell Foundation Druckenmiller fellow and was previously supported by the EMBO European Commission FP7 (Marie Curie Actions; EMBOCOFUND2012, GA-2012-600394, ALTF 447-2014). A.H.Z. was supported by NIH training grant T32 NS007098 and GM007288, and the National Cancer Institute (NCI) predoctoral MD/PhD fellowship F30 CA203446. A.L. is supported by NCI Individual Postdoctoral Fellowship (F32), Ruth L. Kirschstein National Research Service Award (NCI 1F32CA20277). Funding Information: We thank Colette Prophete, Lauren Schiff, and Paul Ciero for technical assistance; the Stem Cell FACS Facility and Einstein Flow Cytometry Core Facility for expert cell sorting; Dr. Toshihide Mizoguchi for advice; and Dr. Wenjun Guo for helpful scientific discussions. This work was supported by R01 grants from the National Institutes of Health ( DK056638 , DK112976 , and HL069438 to P.S.F; DK076602 to M.M.S.), New York State Department of Health on Prostate Cancer Hypothesis Development Research ( C31043GG ) and NYSTEM ( C029570 ). M.H. was supported by a fellowship of the German Research Foundation (DFG, Ha 6731/1-1 ). M.M. is a New York Stem Cell Foundation Druckenmiller fellow and was previously supported by the EMBO European Commission FP7 (Marie Curie Actions; EMBOCOFUND2012 , GA-2012-600394 , ALTF 447-2014 ). A.H.Z. was supported by NIH training grant T32 NS007098 and GM007288 , and the National Cancer Institute (NCI) predoctoral MD/PhD fellowship F30 CA203446 . A.L. is supported by NCI Individual Postdoctoral Fellowship (F32), Ruth L. Kirschstein National Research Service Award (NCI 1F32CA20277 ). Publisher Copyright: {\textcopyright} 2019 The Author(s)",
year = "2019",
month = jun,
day = "11",
doi = "10.1016/j.stemcr.2019.04.019",
language = "English (US)",
volume = "12",
pages = "1201--1211",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "6",
}