Nestin+NG2+ Cells Form a Reserve Stem Cell Population in the Mouse Prostate

Maher Hanoun, Anna Arnal-Estapé, Maria Maryanovich, Ali H. Zahalka, Sarah K. Bergren, Chee W. Chua, Avigdor Leftin, Nils P. Brodin, Michael M. Shen, Chandan Guha, Paul S. Frenette

Research output: Contribution to journalArticle

Abstract

In the prostate, stem and progenitor cell regenerative capacities have been ascribed to both basal and luminal epithelial cells. Here, we show that a rare subset of mesenchymal cells in the prostate are epithelial-primed Nestin-expressing cells (EPNECs) that can generate self-renewing prostate organoids with bipotential capacity. Upon transplantation, these EPNECs can form prostate gland tissue grafts at the clonal level. Lineage-tracing analyses show that cells marked by Nestin or NG2 transgenic mice contribute to prostate epithelium during organogenesis. In the adult, modest contributions in repeated rounds of regression and regeneration are observed, whereas prostate epithelial cells derived from Nestin/NG2-marked cells are dramatically increased after severe irradiation-induced organ damage. These results indicate that Nestin/NG2 expression marks a novel radioresistant prostate stem cell that is active during development and displays reserve stem cell activity for tissue maintenance.

Original languageEnglish (US)
Pages (from-to)1201-1211
Number of pages11
JournalStem Cell Reports
Volume12
Issue number6
DOIs
StatePublished - Jun 11 2019

Fingerprint

Nestin
Stem cells
Prostate
Stem Cells
Population
Tissue
Transplantation (surgical)
Epithelial Cells
Organoids
Grafts
Organogenesis
Irradiation
Transgenic Mice
Regeneration
Epithelium
Transplantation
Maintenance
Transplants

Keywords

  • mesenchymal-to-epithelial transition
  • Nestin
  • prostate stem cell

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Hanoun, M., Arnal-Estapé, A., Maryanovich, M., Zahalka, A. H., Bergren, S. K., Chua, C. W., ... Frenette, P. S. (2019). Nestin+NG2+ Cells Form a Reserve Stem Cell Population in the Mouse Prostate. Stem Cell Reports, 12(6), 1201-1211. https://doi.org/10.1016/j.stemcr.2019.04.019

Nestin+NG2+ Cells Form a Reserve Stem Cell Population in the Mouse Prostate. / Hanoun, Maher; Arnal-Estapé, Anna; Maryanovich, Maria; Zahalka, Ali H.; Bergren, Sarah K.; Chua, Chee W.; Leftin, Avigdor; Brodin, Nils P.; Shen, Michael M.; Guha, Chandan; Frenette, Paul S.

In: Stem Cell Reports, Vol. 12, No. 6, 11.06.2019, p. 1201-1211.

Research output: Contribution to journalArticle

Hanoun, M, Arnal-Estapé, A, Maryanovich, M, Zahalka, AH, Bergren, SK, Chua, CW, Leftin, A, Brodin, NP, Shen, MM, Guha, C & Frenette, PS 2019, 'Nestin+NG2+ Cells Form a Reserve Stem Cell Population in the Mouse Prostate', Stem Cell Reports, vol. 12, no. 6, pp. 1201-1211. https://doi.org/10.1016/j.stemcr.2019.04.019
Hanoun M, Arnal-Estapé A, Maryanovich M, Zahalka AH, Bergren SK, Chua CW et al. Nestin+NG2+ Cells Form a Reserve Stem Cell Population in the Mouse Prostate. Stem Cell Reports. 2019 Jun 11;12(6):1201-1211. https://doi.org/10.1016/j.stemcr.2019.04.019
Hanoun, Maher ; Arnal-Estapé, Anna ; Maryanovich, Maria ; Zahalka, Ali H. ; Bergren, Sarah K. ; Chua, Chee W. ; Leftin, Avigdor ; Brodin, Nils P. ; Shen, Michael M. ; Guha, Chandan ; Frenette, Paul S. / Nestin+NG2+ Cells Form a Reserve Stem Cell Population in the Mouse Prostate. In: Stem Cell Reports. 2019 ; Vol. 12, No. 6. pp. 1201-1211.
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