Abstract
In the prostate, stem and progenitor cell regenerative capacities have been ascribed to both basal and luminal epithelial cells. Here, we show that a rare subset of mesenchymal cells in the prostate are epithelial-primed Nestin-expressing cells (EPNECs) that can generate self-renewing prostate organoids with bipotential capacity. Upon transplantation, these EPNECs can form prostate gland tissue grafts at the clonal level. Lineage-tracing analyses show that cells marked by Nestin or NG2 transgenic mice contribute to prostate epithelium during organogenesis. In the adult, modest contributions in repeated rounds of regression and regeneration are observed, whereas prostate epithelial cells derived from Nestin/NG2-marked cells are dramatically increased after severe irradiation-induced organ damage. These results indicate that Nestin/NG2 expression marks a novel radioresistant prostate stem cell that is active during development and displays reserve stem cell activity for tissue maintenance.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1201-1211 |
| Number of pages | 11 |
| Journal | Stem Cell Reports |
| Volume | 12 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 11 2019 |
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Keywords
- mesenchymal-to-epithelial transition
- Nestin
- prostate stem cell
ASJC Scopus subject areas
- Biochemistry
- Genetics
- Developmental Biology
- Cell Biology
Cite this
Nestin+NG2+ Cells Form a Reserve Stem Cell Population in the Mouse Prostate. / Hanoun, Maher; Arnal-Estapé, Anna; Maryanovich, Maria; Zahalka, Ali H.; Bergren, Sarah K.; Chua, Chee W.; Leftin, Avigdor; Brodin, Patrik N.; Shen, Michael M.; Guha, Chandan; Frenette, Paul S.
In: Stem Cell Reports, Vol. 12, No. 6, 11.06.2019, p. 1201-1211.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nestin+NG2+ Cells Form a Reserve Stem Cell Population in the Mouse Prostate
AU - Hanoun, Maher
AU - Arnal-Estapé, Anna
AU - Maryanovich, Maria
AU - Zahalka, Ali H.
AU - Bergren, Sarah K.
AU - Chua, Chee W.
AU - Leftin, Avigdor
AU - Brodin, Patrik N.
AU - Shen, Michael M.
AU - Guha, Chandan
AU - Frenette, Paul S.
PY - 2019/6/11
Y1 - 2019/6/11
N2 - In the prostate, stem and progenitor cell regenerative capacities have been ascribed to both basal and luminal epithelial cells. Here, we show that a rare subset of mesenchymal cells in the prostate are epithelial-primed Nestin-expressing cells (EPNECs) that can generate self-renewing prostate organoids with bipotential capacity. Upon transplantation, these EPNECs can form prostate gland tissue grafts at the clonal level. Lineage-tracing analyses show that cells marked by Nestin or NG2 transgenic mice contribute to prostate epithelium during organogenesis. In the adult, modest contributions in repeated rounds of regression and regeneration are observed, whereas prostate epithelial cells derived from Nestin/NG2-marked cells are dramatically increased after severe irradiation-induced organ damage. These results indicate that Nestin/NG2 expression marks a novel radioresistant prostate stem cell that is active during development and displays reserve stem cell activity for tissue maintenance.
AB - In the prostate, stem and progenitor cell regenerative capacities have been ascribed to both basal and luminal epithelial cells. Here, we show that a rare subset of mesenchymal cells in the prostate are epithelial-primed Nestin-expressing cells (EPNECs) that can generate self-renewing prostate organoids with bipotential capacity. Upon transplantation, these EPNECs can form prostate gland tissue grafts at the clonal level. Lineage-tracing analyses show that cells marked by Nestin or NG2 transgenic mice contribute to prostate epithelium during organogenesis. In the adult, modest contributions in repeated rounds of regression and regeneration are observed, whereas prostate epithelial cells derived from Nestin/NG2-marked cells are dramatically increased after severe irradiation-induced organ damage. These results indicate that Nestin/NG2 expression marks a novel radioresistant prostate stem cell that is active during development and displays reserve stem cell activity for tissue maintenance.
KW - mesenchymal-to-epithelial transition
KW - Nestin
KW - prostate stem cell
UR - http://www.scopus.com/inward/record.url?scp=85066734295&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85066734295&partnerID=8YFLogxK
U2 - 10.1016/j.stemcr.2019.04.019
DO - 10.1016/j.stemcr.2019.04.019
M3 - Article
C2 - 31130357
AN - SCOPUS:85066734295
VL - 12
SP - 1201
EP - 1211
JO - Stem Cell Reports
JF - Stem Cell Reports
SN - 2213-6711
IS - 6
ER -