Nestin expression in pancreatic endocrine and exocrine cells of mice lacking glucagon signaling

Mamdouh H. Kedees, Yelena Guz, Patricia M. Vuguin, Carlos Vargas, Lingguang Cui, Donald F. Steiner, Maureen J. Charron, Gladys Teitelman

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Nestin, a marker of neural stem cells, is also expressed by cells located in the epithelium of the pancreatic primordium and by a subpopulation of exocrine cells but not by endocrine cells. These findings raised the possibility that the pancreatic epithelium is heterogeneous and comprised of subpopulations of exocrine/nestin-positive and endocrine/nestin-negative precursor cells. We examined this issue in two mutant mouse models characterized by protracted expression of several embryonal properties in islet cells. One mutant line comprises mice lacking mature glucagon due to abrogation of proprotein convertase-2 (PC2-/-), responsible for the conversion of proglucagon into glucagon, while the second line consists of mice with a global deletion of the glucagon receptor (Gcgr_/_). We demonstrate that nestin is transiently expressed by acinar cells and by insulin and glucagon cells of islets of both lines of mice. In addition, the lack of glucagon signaling increased nestin mRNA levels in pancreas of mutant embryos and adult mice. We conclude that nestin+ cells located in the pancreatic primordium generate the cells of the endocrine and exocrine lineages. Furthermore, our results suggest that nestin expression is regulated by glucagon signaling.

Original languageEnglish (US)
Pages (from-to)1126-1133
Number of pages8
JournalDevelopmental Dynamics
Volume236
Issue number4
DOIs
StatePublished - Apr 2007

Fingerprint

Nestin
Endocrine Cells
Glucagon
Islets of Langerhans
Proprotein Convertase 2
Epithelium
Proglucagon
Glucagon Receptors
Neural Stem Cells
Acinar Cells
Pancreas
Embryonic Structures
Insulin
Messenger RNA

Keywords

  • Glucagon receptor
  • Glucagon signaling
  • Mouse development
  • Nestin
  • Pancreatic islet cells
  • Proprotein convertases

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Kedees, M. H., Guz, Y., Vuguin, P. M., Vargas, C., Cui, L., Steiner, D. F., ... Teitelman, G. (2007). Nestin expression in pancreatic endocrine and exocrine cells of mice lacking glucagon signaling. Developmental Dynamics, 236(4), 1126-1133. https://doi.org/10.1002/dvdy.21112

Nestin expression in pancreatic endocrine and exocrine cells of mice lacking glucagon signaling. / Kedees, Mamdouh H.; Guz, Yelena; Vuguin, Patricia M.; Vargas, Carlos; Cui, Lingguang; Steiner, Donald F.; Charron, Maureen J.; Teitelman, Gladys.

In: Developmental Dynamics, Vol. 236, No. 4, 04.2007, p. 1126-1133.

Research output: Contribution to journalArticle

Kedees, MH, Guz, Y, Vuguin, PM, Vargas, C, Cui, L, Steiner, DF, Charron, MJ & Teitelman, G 2007, 'Nestin expression in pancreatic endocrine and exocrine cells of mice lacking glucagon signaling', Developmental Dynamics, vol. 236, no. 4, pp. 1126-1133. https://doi.org/10.1002/dvdy.21112
Kedees, Mamdouh H. ; Guz, Yelena ; Vuguin, Patricia M. ; Vargas, Carlos ; Cui, Lingguang ; Steiner, Donald F. ; Charron, Maureen J. ; Teitelman, Gladys. / Nestin expression in pancreatic endocrine and exocrine cells of mice lacking glucagon signaling. In: Developmental Dynamics. 2007 ; Vol. 236, No. 4. pp. 1126-1133.
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