Neo-epitopes on crotonaldehyde modified DNA preferably recognize circulating autoantibodies in cancer patients

Badar ul Islam, Parvez Ahmad, Gulam Rabbani, Kiran Dixit, Moinuddin, Shahid Ali Siddiqui, Asif Ali

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

DNA damage is one of the leading causes of various pathological conditions including carcinogenesis. Crotonaldehyde is a 4-carbon unsaturated bifunctional aldehyde which is found ubiquitously and produced both exogenously and endogenously. It reacts with deoxyguanosine and form adducts with DNA. These adducts were detected and found involved in tumor formation in rats treated with crotonaldehyde. In the present study, structural changes in DNA by crotonaldehyde were evaluated by Fourier transform infrared (FTIR) spectroscopy, differential scanning colorimetry (DSC), dynamic light scattering (DLS), high-performance liquid chromatography (HPLC), and atomic force microscopy (AFM). Enhanced binding was observed in cancer autoantibodies with the DNA modified by crotonaldehyde than the native counterpart. Immunological studies revealed enhanced binding of cancer autoantibodies with crotonaldehyde modified DNA, compared to the native form. Furthermore, lymphocyte DNA isolated from cancer patients demonstrated considerable recognition of anti-Cro-DNA IgG as compared to the DNA from healthy individuals. Therefore, we suggest that crotonaldehyde modified DNA presents unique epitopes, that may trigger autoantibody induction in cancer patients.

Original languageEnglish (US)
Pages (from-to)1817-1824
Number of pages8
JournalTumor Biology
Volume37
Issue number2
DOIs
StatePublished - Feb 1 2016
Externally publishedYes

Keywords

  • Cancer
  • Crosslinking
  • Crotonaldehyde
  • Human DNA
  • Neo-epitopes

ASJC Scopus subject areas

  • Cancer Research

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