TY - JOUR
T1 - Negative selection of thymocytes
T2 - A novel polymerase chain reaction-based molecular analysis detects requirements for macromolecular synthesis
AU - D'Adamio, Luciano
AU - Clayton, Linda K.
AU - Awad, Katherine M.
AU - Reinherz, Ellis L.
PY - 1992
Y1 - 1992
N2 - Self-tolerance is mainly established through clonal deletion of autoreactive T cells during thymic differentiation. The mechanisms by which deletion is achieved are poorly understood. Here we use a specific polymerase chain reaction-based system to characterize DNA fragmentation and show that after in vivo treatment of neonatal mice with staphylococcus enterotoxin B, selective apoptosis of Vβ8+ thymocytes occurs. This process precedes detectable deletion of Vβ8+ cells as determined by phenotypic analysis. Moreover, in vivo administration of cycloheximide and, to a lesser extent, actinomycin D, inhibits apoptosis of staphylococcus enterotoxin B specific thymocytes. Thus, macromolecular synthesis is a requirement for negative selection.
AB - Self-tolerance is mainly established through clonal deletion of autoreactive T cells during thymic differentiation. The mechanisms by which deletion is achieved are poorly understood. Here we use a specific polymerase chain reaction-based system to characterize DNA fragmentation and show that after in vivo treatment of neonatal mice with staphylococcus enterotoxin B, selective apoptosis of Vβ8+ thymocytes occurs. This process precedes detectable deletion of Vβ8+ cells as determined by phenotypic analysis. Moreover, in vivo administration of cycloheximide and, to a lesser extent, actinomycin D, inhibits apoptosis of staphylococcus enterotoxin B specific thymocytes. Thus, macromolecular synthesis is a requirement for negative selection.
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M3 - Article
C2 - 1331238
AN - SCOPUS:0026457329
SN - 0022-1767
VL - 149
SP - 3550
EP - 3553
JO - Journal of Immunology
JF - Journal of Immunology
IS - 11
ER -