Negative selection of thymocytes: A novel polymerase chain reaction-based molecular analysis detects requirements for macromolecular synthesis

Luciano D'Adamio, Linda K. Clayton, Katherine M. Awad, Ellis L. Reinherz

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Self-tolerance is mainly established through clonal deletion of autoreactive T cells during thymic differentiation. The mechanisms by which deletion is achieved are poorly understood. Here we use a specific polymerase chain reaction-based system to characterize DNA fragmentation and show that after in vivo treatment of neonatal mice with staphylococcus enterotoxin B, selective apoptosis of Vβ8+ thymocytes occurs. This process precedes detectable deletion of Vβ8+ cells as determined by phenotypic analysis. Moreover, in vivo administration of cycloheximide and, to a lesser extent, actinomycin D, inhibits apoptosis of staphylococcus enterotoxin B specific thymocytes. Thus, macromolecular synthesis is a requirement for negative selection.

Original languageEnglish (US)
Pages (from-to)3550-3553
Number of pages4
JournalJournal of Immunology
Volume149
Issue number11
StatePublished - 1992
Externally publishedYes

Fingerprint

Thymocytes
Clonal Deletion
Apoptosis
Self Tolerance
Polymerase Chain Reaction
Dactinomycin
DNA Fragmentation
Cycloheximide
T-Lymphocytes
staphylococcal enterotoxin B
Therapeutics

ASJC Scopus subject areas

  • Immunology

Cite this

Negative selection of thymocytes : A novel polymerase chain reaction-based molecular analysis detects requirements for macromolecular synthesis. / D'Adamio, Luciano; Clayton, Linda K.; Awad, Katherine M.; Reinherz, Ellis L.

In: Journal of Immunology, Vol. 149, No. 11, 1992, p. 3550-3553.

Research output: Contribution to journalArticle

D'Adamio, Luciano ; Clayton, Linda K. ; Awad, Katherine M. ; Reinherz, Ellis L. / Negative selection of thymocytes : A novel polymerase chain reaction-based molecular analysis detects requirements for macromolecular synthesis. In: Journal of Immunology. 1992 ; Vol. 149, No. 11. pp. 3550-3553.
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