Negative feedback regulation of Wnt signaling via N-linked fucosylation in zebrafish

Lei Feng, Hao Jiang, Peng Wu, Florence L. Marlow

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

L-fucose, a monosaccharide widely distributed in eukaryotes and certain bacteria, is a determinant of many functional glycans that play central roles in numerous biological processes. The molecular mechanism, however, by which fucosylation mediates these processes remains largely elusive. To study how changes in fucosylation impact embryonic development, we up-regulated N-linked fucosylation via over-expression of a key GDP-Fucose transporter, Slc35c1, in zebrafish. We show that Slc35c1 overexpression causes elevated N-linked fucosylation and disrupts embryonic patterning in a transporter activity dependent manner. We demonstrate that patterning defects associated with enhanced N-linked fucosylation are due to diminished canonical Wnt signaling. Chimeric analyses demonstrate that elevated Slc35c1 expression in receiving cells decreases the signaling range of Wnt8a during zebrafish embryogenesis. Moreover, we provide biochemical evidence that this decrease is associated with reduced Wnt8 ligand and elevated Lrp6 coreceptor, which we show are both substrates for N-linked fucosylation in zebrafish embryos. Strikingly, slc35c1 expression is regulated by canonical Wnt signaling. These results suggest that Wnt limits its own signaling activity in part via up-regulation of a transporter, slc35c1 that promotes terminal fucosylation and thereby limits Wnt activity.

Original languageEnglish (US)
Pages (from-to)268-286
Number of pages19
JournalDevelopmental Biology
Volume395
Issue number2
DOIs
StatePublished - Nov 15 2014

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Zebrafish
Embryonic Development
Guanosine Diphosphate Fucose
Biological Phenomena
Fucose
Monosaccharides
Eukaryota
Polysaccharides
Up-Regulation
Embryonic Structures
Ligands
Bacteria

Keywords

  • Fucosylation
  • GDP-Fucose transporter
  • Slc35c1
  • Wnt signaling
  • Zebrafish patterning

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Negative feedback regulation of Wnt signaling via N-linked fucosylation in zebrafish. / Feng, Lei; Jiang, Hao; Wu, Peng; Marlow, Florence L.

In: Developmental Biology, Vol. 395, No. 2, 15.11.2014, p. 268-286.

Research output: Contribution to journalArticle

Feng, Lei ; Jiang, Hao ; Wu, Peng ; Marlow, Florence L. / Negative feedback regulation of Wnt signaling via N-linked fucosylation in zebrafish. In: Developmental Biology. 2014 ; Vol. 395, No. 2. pp. 268-286.
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