Near-infrared fluorescent proteins engineered from bacterial phytochromes

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Near-infrared fluorescent proteins (NIR FPs), photoactivatable NIR FPs and NIR reporters of protein-protein interactions developed from bacterial phytochrome photoreceptors (BphPs) have advanced non-invasive deep-tissue imaging. Here we provide a brief guide to the BphP-derived NIR probes with an emphasis on their in vivo applications. We describe phenotypes of NIR FPs and their photochemical and intracellular properties. We discuss NIR FP applications for imaging of various cell types, tissues and animal models in basic and translational research. In this discussion, we focus on NIR FPs that efficiently incorporate endogenous biliverdin chromophore and therefore can be used as straightforward as GFP-like proteins. We also overview a usage of NIR FPs in different imaging platforms, from planar epifluorescence to tomographic and photoacoustic technologies.

Original languageEnglish (US)
Pages (from-to)52-63
Number of pages12
JournalCurrent Opinion in Chemical Biology
Volume27
DOIs
StatePublished - Aug 1 2015

Fingerprint

Phytochrome
Bacterial Proteins
Infrared radiation
Proteins
Imaging techniques
Photoacoustic Techniques
Biliverdine
Tissue
Translational Medical Research
Photoacoustic effect
Chromophores
Animals
Animal Models
Phenotype

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry

Cite this

Near-infrared fluorescent proteins engineered from bacterial phytochromes. / Shcherbakova, Daria; Baloban, Mikhail; Verkhusha, Vladislav.

In: Current Opinion in Chemical Biology, Vol. 27, 01.08.2015, p. 52-63.

Research output: Contribution to journalArticle

@article{d97d12423067467f9918c1d62df8360c,
title = "Near-infrared fluorescent proteins engineered from bacterial phytochromes",
abstract = "Near-infrared fluorescent proteins (NIR FPs), photoactivatable NIR FPs and NIR reporters of protein-protein interactions developed from bacterial phytochrome photoreceptors (BphPs) have advanced non-invasive deep-tissue imaging. Here we provide a brief guide to the BphP-derived NIR probes with an emphasis on their in vivo applications. We describe phenotypes of NIR FPs and their photochemical and intracellular properties. We discuss NIR FP applications for imaging of various cell types, tissues and animal models in basic and translational research. In this discussion, we focus on NIR FPs that efficiently incorporate endogenous biliverdin chromophore and therefore can be used as straightforward as GFP-like proteins. We also overview a usage of NIR FPs in different imaging platforms, from planar epifluorescence to tomographic and photoacoustic technologies.",
author = "Daria Shcherbakova and Mikhail Baloban and Vladislav Verkhusha",
year = "2015",
month = "8",
day = "1",
doi = "10.1016/j.cbpa.2015.06.005",
language = "English (US)",
volume = "27",
pages = "52--63",
journal = "Current Opinion in Chemical Biology",
issn = "1367-5931",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Near-infrared fluorescent proteins engineered from bacterial phytochromes

AU - Shcherbakova, Daria

AU - Baloban, Mikhail

AU - Verkhusha, Vladislav

PY - 2015/8/1

Y1 - 2015/8/1

N2 - Near-infrared fluorescent proteins (NIR FPs), photoactivatable NIR FPs and NIR reporters of protein-protein interactions developed from bacterial phytochrome photoreceptors (BphPs) have advanced non-invasive deep-tissue imaging. Here we provide a brief guide to the BphP-derived NIR probes with an emphasis on their in vivo applications. We describe phenotypes of NIR FPs and their photochemical and intracellular properties. We discuss NIR FP applications for imaging of various cell types, tissues and animal models in basic and translational research. In this discussion, we focus on NIR FPs that efficiently incorporate endogenous biliverdin chromophore and therefore can be used as straightforward as GFP-like proteins. We also overview a usage of NIR FPs in different imaging platforms, from planar epifluorescence to tomographic and photoacoustic technologies.

AB - Near-infrared fluorescent proteins (NIR FPs), photoactivatable NIR FPs and NIR reporters of protein-protein interactions developed from bacterial phytochrome photoreceptors (BphPs) have advanced non-invasive deep-tissue imaging. Here we provide a brief guide to the BphP-derived NIR probes with an emphasis on their in vivo applications. We describe phenotypes of NIR FPs and their photochemical and intracellular properties. We discuss NIR FP applications for imaging of various cell types, tissues and animal models in basic and translational research. In this discussion, we focus on NIR FPs that efficiently incorporate endogenous biliverdin chromophore and therefore can be used as straightforward as GFP-like proteins. We also overview a usage of NIR FPs in different imaging platforms, from planar epifluorescence to tomographic and photoacoustic technologies.

UR - http://www.scopus.com/inward/record.url?scp=84935005822&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84935005822&partnerID=8YFLogxK

U2 - 10.1016/j.cbpa.2015.06.005

DO - 10.1016/j.cbpa.2015.06.005

M3 - Article

C2 - 26115447

AN - SCOPUS:84935005822

VL - 27

SP - 52

EP - 63

JO - Current Opinion in Chemical Biology

JF - Current Opinion in Chemical Biology

SN - 1367-5931

ER -