Near-Infrared Fluorescent Proteins and Their Applications

M. M. Karasev, O. V. Stepanenko, K. A. Rumyantsev, K. K. Turoverov, Vladislav Verkhusha

Research output: Contribution to journalReview article

1 Citation (Scopus)

Abstract

High transparency, low light-scattering, and low autofluorescence of mammalian tissues in the near-infrared (NIR) spectral range (~650–900 nm) open a possibility for in vivo imaging of biological processes at the micro-and macroscales to address basic and applied problems in biology and biomedicine. Recently, probes that absorb and fluoresce in the NIR optical range have been engineered using bacterial phytochromes–natural NIR light-absorbing photoreceptors that regulate metabolism in bacteria. Since the chromophore in all these proteins is biliverdin, a natural product of heme catabolism in mammalian cells, they can be used as genetically encoded fluorescent probes, similarly to GFP-like fluorescent proteins. In this review, we discuss photophysical and biochemical properties of NIR fluorescent proteins, reporters, and biosensors and analyze their characteristics required for expression of these molecules in mammalian cells. Structural features and molecular engineering of NIR fluorescent probes are discussed. Applications of NIR fluorescent proteins and biosensors for studies of molecular processes in cells, as well as for tissue and organ visualization in whole-body imaging in vivo, are described. We specifically focus on the use of NIR fluorescent probes in advanced imaging technologies that combine fluorescence and bioluminescence methods with photoacoustic tomography.

Original languageEnglish (US)
Pages (from-to)32-50
Number of pages19
JournalBiochemistry (Moscow)
Volume84
DOIs
StatePublished - Jan 1 2019

Fingerprint

Fluorescent Dyes
Infrared radiation
Biosensing Techniques
Proteins
Biliverdine
Biological Phenomena
Whole Body Imaging
Light
Imaging techniques
Biosensors
Biological Products
Heme
Cells
Tissue
Bioluminescence
Fluorescence
Tomography
Technology
Bacteria
Photoacoustic effect

Keywords

  • bacterial phytochromes
  • bioluminescence
  • biomarkers
  • biosensors
  • fluorescence
  • fluorescent proteins
  • in vivo visualization

ASJC Scopus subject areas

  • Biochemistry

Cite this

Karasev, M. M., Stepanenko, O. V., Rumyantsev, K. A., Turoverov, K. K., & Verkhusha, V. (2019). Near-Infrared Fluorescent Proteins and Their Applications. Biochemistry (Moscow), 84, 32-50. https://doi.org/10.1134/S0006297919140037

Near-Infrared Fluorescent Proteins and Their Applications. / Karasev, M. M.; Stepanenko, O. V.; Rumyantsev, K. A.; Turoverov, K. K.; Verkhusha, Vladislav.

In: Biochemistry (Moscow), Vol. 84, 01.01.2019, p. 32-50.

Research output: Contribution to journalReview article

Karasev, MM, Stepanenko, OV, Rumyantsev, KA, Turoverov, KK & Verkhusha, V 2019, 'Near-Infrared Fluorescent Proteins and Their Applications', Biochemistry (Moscow), vol. 84, pp. 32-50. https://doi.org/10.1134/S0006297919140037
Karasev, M. M. ; Stepanenko, O. V. ; Rumyantsev, K. A. ; Turoverov, K. K. ; Verkhusha, Vladislav. / Near-Infrared Fluorescent Proteins and Their Applications. In: Biochemistry (Moscow). 2019 ; Vol. 84. pp. 32-50.
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