Natural photoreceptors as a source of fluorescent proteins, biosensors, and optogenetic tools

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Genetically encoded optical tools have revolutionized modern biology by allowing detection and control of biological processes with exceptional spatiotemporal precision and sensitivity. Natural photoreceptors provide researchers with a vast source of molecular templates for engineering of fluorescent proteins, biosensors, and optogenetic tools. Here, we give a brief overview of natural photoreceptors and their mechanisms of action. We then discuss fluorescent proteins and biosensors developed from light-oxygen-voltage-sensing (LOV) domains and phytochromes, as well as their properties and applications. These fluorescent tools possess unique characteristics not achievable with green fluorescent protein-like probes, including near-infrared fluorescence, independence of oxygen, small size, and photosensitizer activity. We next provide an overview of available optogenetic tools of various origins, such as LOV and BLUF (blue-light-utilizing flavin adenine dinucleotide) domains, cryptochromes, and phytochromes, enabling control of versatile cellular processes. We analyze the principles of their function and practical requirements for use. We focus mainly on optical tools with demonstrated use beyond bacteria, with a specific emphasis on their applications in mammalian cells.

Original languageEnglish (US)
Pages (from-to)519-550
Number of pages32
JournalAnnual Review of Biochemistry
Volume84
DOIs
StatePublished - Jun 2 2015

Fingerprint

Optogenetics
Biosensing Techniques
Biosensors
Phytochrome
Oxygen
Light
Cryptochromes
Biological Phenomena
Protein Engineering
Proteins
Flavin-Adenine Dinucleotide
Photosensitizing Agents
Green Fluorescent Proteins
Fluorescence
Research Personnel
Bacteria
Electric potential
Cells
Infrared radiation

Keywords

  • Bacteriophytochrome
  • BphP
  • CRY2
  • IRFP
  • LOV domain
  • Optogenetics
  • Phytochrome

ASJC Scopus subject areas

  • Biochemistry

Cite this

@article{9a6e8bdcadb842efa0e19dd107137134,
title = "Natural photoreceptors as a source of fluorescent proteins, biosensors, and optogenetic tools",
abstract = "Genetically encoded optical tools have revolutionized modern biology by allowing detection and control of biological processes with exceptional spatiotemporal precision and sensitivity. Natural photoreceptors provide researchers with a vast source of molecular templates for engineering of fluorescent proteins, biosensors, and optogenetic tools. Here, we give a brief overview of natural photoreceptors and their mechanisms of action. We then discuss fluorescent proteins and biosensors developed from light-oxygen-voltage-sensing (LOV) domains and phytochromes, as well as their properties and applications. These fluorescent tools possess unique characteristics not achievable with green fluorescent protein-like probes, including near-infrared fluorescence, independence of oxygen, small size, and photosensitizer activity. We next provide an overview of available optogenetic tools of various origins, such as LOV and BLUF (blue-light-utilizing flavin adenine dinucleotide) domains, cryptochromes, and phytochromes, enabling control of versatile cellular processes. We analyze the principles of their function and practical requirements for use. We focus mainly on optical tools with demonstrated use beyond bacteria, with a specific emphasis on their applications in mammalian cells.",
keywords = "Bacteriophytochrome, BphP, CRY2, IRFP, LOV domain, Optogenetics, Phytochrome",
author = "Daria Shcherbakova and Anton Shemetov and Andrii Kaberniuk and Vladislav Verkhusha",
year = "2015",
month = "6",
day = "2",
doi = "10.1146/annurev-biochem-060614-034411",
language = "English (US)",
volume = "84",
pages = "519--550",
journal = "Annual Review of Biochemistry",
issn = "0066-4154",
publisher = "Annual Reviews Inc.",

}

TY - JOUR

T1 - Natural photoreceptors as a source of fluorescent proteins, biosensors, and optogenetic tools

AU - Shcherbakova, Daria

AU - Shemetov, Anton

AU - Kaberniuk, Andrii

AU - Verkhusha, Vladislav

PY - 2015/6/2

Y1 - 2015/6/2

N2 - Genetically encoded optical tools have revolutionized modern biology by allowing detection and control of biological processes with exceptional spatiotemporal precision and sensitivity. Natural photoreceptors provide researchers with a vast source of molecular templates for engineering of fluorescent proteins, biosensors, and optogenetic tools. Here, we give a brief overview of natural photoreceptors and their mechanisms of action. We then discuss fluorescent proteins and biosensors developed from light-oxygen-voltage-sensing (LOV) domains and phytochromes, as well as their properties and applications. These fluorescent tools possess unique characteristics not achievable with green fluorescent protein-like probes, including near-infrared fluorescence, independence of oxygen, small size, and photosensitizer activity. We next provide an overview of available optogenetic tools of various origins, such as LOV and BLUF (blue-light-utilizing flavin adenine dinucleotide) domains, cryptochromes, and phytochromes, enabling control of versatile cellular processes. We analyze the principles of their function and practical requirements for use. We focus mainly on optical tools with demonstrated use beyond bacteria, with a specific emphasis on their applications in mammalian cells.

AB - Genetically encoded optical tools have revolutionized modern biology by allowing detection and control of biological processes with exceptional spatiotemporal precision and sensitivity. Natural photoreceptors provide researchers with a vast source of molecular templates for engineering of fluorescent proteins, biosensors, and optogenetic tools. Here, we give a brief overview of natural photoreceptors and their mechanisms of action. We then discuss fluorescent proteins and biosensors developed from light-oxygen-voltage-sensing (LOV) domains and phytochromes, as well as their properties and applications. These fluorescent tools possess unique characteristics not achievable with green fluorescent protein-like probes, including near-infrared fluorescence, independence of oxygen, small size, and photosensitizer activity. We next provide an overview of available optogenetic tools of various origins, such as LOV and BLUF (blue-light-utilizing flavin adenine dinucleotide) domains, cryptochromes, and phytochromes, enabling control of versatile cellular processes. We analyze the principles of their function and practical requirements for use. We focus mainly on optical tools with demonstrated use beyond bacteria, with a specific emphasis on their applications in mammalian cells.

KW - Bacteriophytochrome

KW - BphP

KW - CRY2

KW - IRFP

KW - LOV domain

KW - Optogenetics

KW - Phytochrome

UR - http://www.scopus.com/inward/record.url?scp=84930732185&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84930732185&partnerID=8YFLogxK

U2 - 10.1146/annurev-biochem-060614-034411

DO - 10.1146/annurev-biochem-060614-034411

M3 - Article

VL - 84

SP - 519

EP - 550

JO - Annual Review of Biochemistry

JF - Annual Review of Biochemistry

SN - 0066-4154

ER -