Nanomolar nitric oxide concentrations quickly and reversibly modulate astrocytic energy metabolism

Alejandro San Martín, Robinson Arce-Molina, Alex Galaz, Gustavo Pérez-Guerra, L. Felipe Barros, Jeffrey E. Pessin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Nitric oxide (NO) is an intercellular messenger involved in multiple bodily functions. Prolonged NO exposure irreversibly inhibits respiration by covalent modification of mitochondrial cytochrome oxidase, a phenomenon of pathological relevance. However, the speed and potency of NO's metabolic effects at physiological concentrations are incompletely characterized. To this end, we set out to investigate the metabolic effects of NO in cultured astrocytes from mice by taking advantage of the high spatiotemporal resolution afforded by genetically encoded Förster resonance energy transfer (FRET) nanosensors. NO exposure resulted in immediate and reversible intracellular glucose depletion and lactate accumulation. Consistent with cyto-chrome oxidase involvement, the glycolytic effect was enhanced at a low oxygen level and became irreversible at a high NO concentration or after prolonged exposure. Measurements of both glycolytic rate and mitochondrial pyruvate consumption revealed significant effects even at nanomolar NO concentrations. We conclude that NO can modulate astrocytic energy metabolism in the short term, reversibly, and at concentrations known to be released by endothelial cells under physiological conditions. These findings suggest that NO modulates the size of the astrocytic lactate reservoir involved in neuronal fueling and signaling.

Original languageEnglish (US)
Pages (from-to)9432-9438
Number of pages7
JournalJournal of Biological Chemistry
Volume292
Issue number22
DOIs
StatePublished - Jun 2 2017

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Energy Metabolism
Nitric Oxide
Lactic Acid
Nanosensors
Fueling
Energy Transfer
Endothelial cells
Electron Transport Complex IV
Pyruvic Acid
Astrocytes
Energy transfer
Oxidoreductases
Respiration
Endothelial Cells
Oxygen
Glucose

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Nanomolar nitric oxide concentrations quickly and reversibly modulate astrocytic energy metabolism. / Martín, Alejandro San; Arce-Molina, Robinson; Galaz, Alex; Pérez-Guerra, Gustavo; Barros, L. Felipe; Pessin, Jeffrey E.

In: Journal of Biological Chemistry, Vol. 292, No. 22, 02.06.2017, p. 9432-9438.

Research output: Contribution to journalArticle

Martín, Alejandro San ; Arce-Molina, Robinson ; Galaz, Alex ; Pérez-Guerra, Gustavo ; Barros, L. Felipe ; Pessin, Jeffrey E. / Nanomolar nitric oxide concentrations quickly and reversibly modulate astrocytic energy metabolism. In: Journal of Biological Chemistry. 2017 ; Vol. 292, No. 22. pp. 9432-9438.
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