Naked mole-rat has increased translational fidelity compared with the mouse, as well as a unique 28S ribosomal RNA cleavage

Jorge Azpurua, Zhonghe Ke, Iris X. Chen, Quanwei Zhang, Dmitri N. Ermolenko, Zhengdong Zhang, Vera Gorbunova, Andrei Seluanov

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The naked mole-rat (Heterocephalus glaber) is a subterranean eusocial rodent with a markedly long lifespan and resistance to tumorigenesis. Multiple data implicate modulation of protein translation in longevity. Here we report that 28S ribosomal RNA (rRNA) of the naked mole-rat is processed into two smaller fragments of unequal size. The two breakpoints are located in the 28S rRNA divergent region 6 and excise a fragment of 263 nt. The excised fragment is unique to the naked mole-rat rRNA and does not show homology to other genomic regions. Because this hidden break site could alter ribosome structure, we investigated whether translation rate and amino acid incorporation fidelity were altered. We report that naked mole-rat fibroblasts have significantly increased translational fidelity despite having comparable translation rates with mouse fibroblasts. Although we cannot directly test whether the unique 28S rRNA structure contributes to the increased fidelity of translation, we speculate that it may change the folding or dynamics of the large ribosomal subunit, altering the rate of GTP hydrolysis and/or interaction of the large subunit with tRNA during accommodation, thus affecting the fidelity of protein synthesis. In summary, our results show that naked mole-rat cells produce fewer aberrant proteins, supporting the hypothesis that the more stable proteome of the naked mole-rat contributes to its longevity.

Original languageEnglish (US)
Pages (from-to)17350-17355
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number43
DOIs
StatePublished - Oct 22 2013

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Mole Rats
28S Ribosomal RNA
Fibroblasts
Large Ribosome Subunits
Ribosomal RNA
Protein Biosynthesis
Proteome
Transfer RNA
Guanosine Triphosphate
Ribosomes
Rodentia
Carcinogenesis
Proteins
Hydrolysis
Amino Acids

Keywords

  • Aging
  • NMR

ASJC Scopus subject areas

  • General

Cite this

Naked mole-rat has increased translational fidelity compared with the mouse, as well as a unique 28S ribosomal RNA cleavage. / Azpurua, Jorge; Ke, Zhonghe; Chen, Iris X.; Zhang, Quanwei; Ermolenko, Dmitri N.; Zhang, Zhengdong; Gorbunova, Vera; Seluanov, Andrei.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 43, 22.10.2013, p. 17350-17355.

Research output: Contribution to journalArticle

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