NAD+ supplementation attenuates methylmercury dopaminergic and mitochondrial toxicity in Caenorhabditis elegans

Samuel W. Caito, Michael Aschner

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Methylmercury (MeHg) is a neurotoxic contaminant of our fish supply that has been linked to dopaminergic (DAergic) dysfunction that characterizes Parkinson's disease. We have previously shown that MeHg causes both morphological and behavioral changes in the Caenorhabditis elegans DAergic neurons that are associated with oxidative stress. We were therefore interested in whether the redox sensitive cofactor nicotinamide adenine dinucleotide (NAD+) may be affected by MeHg and whether supplementation of NAD+may prevent MeHg-induced toxicities. Worms treated with MeHg showed depletion in cellular NAD+levels, which was prevented by NAD+supplementation prior to MeHg treatment. NAD+supplementation also prevented DAergic neurodegeneration and deficits in DAergic-dependent behavior upon MeHg exposure. In a mutant worm line that cannot synthesize NAD+from nicotinamide, MeHg lethality and DAergic behavioral deficits were more sensitive to MeHg than wildtype worms, demonstrating the importance of NAD+in MeHg toxicity. In wildtype worms, NAD+supplementation provided protection from MeHg-induced oxidative stress and mitochondrial dysfunction. These data show the importance of NAD+levels in the response to MeHg exposure. NAD+supplementation may be beneficial for MeHg-induced toxicities and preventing cellular damage involved in Parkinson's disease.

Original languageEnglish (US)
Pages (from-to)139-149
Number of pages11
JournalToxicological Sciences
Volume151
Issue number1
DOIs
StatePublished - May 1 2016

Fingerprint

Caenorhabditis elegans
NAD
Toxicity
Oxidative stress
Parkinson Disease
Oxidative Stress
Niacinamide
Dopaminergic Neurons
Fish
Neurons
Oxidation-Reduction
Fishes
Impurities

Keywords

  • C. elegans
  • Dopaminergic neurons
  • Methylmercury
  • Mitochondria

ASJC Scopus subject areas

  • Toxicology

Cite this

NAD+ supplementation attenuates methylmercury dopaminergic and mitochondrial toxicity in Caenorhabditis elegans. / Caito, Samuel W.; Aschner, Michael.

In: Toxicological Sciences, Vol. 151, No. 1, 01.05.2016, p. 139-149.

Research output: Contribution to journalArticle

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