N-Methyl-D-Aspartic Acid Receptors on Striatal Neurons Are Essential for Cocaine Cue Reactivity in Mice

Soh Agatsuma, Mai T. Dang, Yuqing Li, Noboru Hiroi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: Environmental cues associated with cocaine evoke craving and seeking. This process, termed cue reactivity, is a critical element of cocaine addiction. Although glutamatergic neurotransmission has been implicated in this effect of cocaine, the precise subtype and localization in the brain of the glutamatergic receptor critical for cocaine cue reactivity is not well-understood. Methods: We used a conditional N-methyl-D-aspartic acid receptor (NMDAR) knockout mouse whose NMDAR gene was deleted by Cre expression restricted to striatal neurons. To evaluate the role of NMDAR in cocaine cue reactivity, conditional knockout mice and control mice (n = 5-8/group) were conditioned for place preference with cocaine (5 and 10 mg/kg SC) for 3 days. Their subsequent place preference was examined in a drug-free state. Results: Although control mice developed cocaine conditioned place preference, mice deficient for NMDAR on striatal neurons failed to develop conditioned place preference. Conclusions: The NMDAR on striatal neurons is essential for the development of cocaine cue reactivity in the place conditioning paradigm. Our finding identifies a brain region whose constitutive NMDAR level serves as a determinant for susceptibility to this aspect of cocaine addiction.

Original languageEnglish (US)
Pages (from-to)778-780
Number of pages3
JournalBiological Psychiatry
Volume67
Issue number8
DOIs
StatePublished - Apr 15 2010

Fingerprint

Corpus Striatum
N-Methylaspartate
Cocaine
Cues
Neurons
Cocaine-Related Disorders
Knockout Mice
Brain
Synaptic Transmission
aspartic acid receptor
Pharmaceutical Preparations
Genes

Keywords

  • Addiction
  • cocaine
  • conditional knockout mouse
  • conditioned place preference
  • cue reactivity
  • striatum

ASJC Scopus subject areas

  • Biological Psychiatry

Cite this

N-Methyl-D-Aspartic Acid Receptors on Striatal Neurons Are Essential for Cocaine Cue Reactivity in Mice. / Agatsuma, Soh; Dang, Mai T.; Li, Yuqing; Hiroi, Noboru.

In: Biological Psychiatry, Vol. 67, No. 8, 15.04.2010, p. 778-780.

Research output: Contribution to journalArticle

Agatsuma, Soh ; Dang, Mai T. ; Li, Yuqing ; Hiroi, Noboru. / N-Methyl-D-Aspartic Acid Receptors on Striatal Neurons Are Essential for Cocaine Cue Reactivity in Mice. In: Biological Psychiatry. 2010 ; Vol. 67, No. 8. pp. 778-780.
@article{1ed6719e6ab440fa9cb7718aeb67d5df,
title = "N-Methyl-D-Aspartic Acid Receptors on Striatal Neurons Are Essential for Cocaine Cue Reactivity in Mice",
abstract = "Background: Environmental cues associated with cocaine evoke craving and seeking. This process, termed cue reactivity, is a critical element of cocaine addiction. Although glutamatergic neurotransmission has been implicated in this effect of cocaine, the precise subtype and localization in the brain of the glutamatergic receptor critical for cocaine cue reactivity is not well-understood. Methods: We used a conditional N-methyl-D-aspartic acid receptor (NMDAR) knockout mouse whose NMDAR gene was deleted by Cre expression restricted to striatal neurons. To evaluate the role of NMDAR in cocaine cue reactivity, conditional knockout mice and control mice (n = 5-8/group) were conditioned for place preference with cocaine (5 and 10 mg/kg SC) for 3 days. Their subsequent place preference was examined in a drug-free state. Results: Although control mice developed cocaine conditioned place preference, mice deficient for NMDAR on striatal neurons failed to develop conditioned place preference. Conclusions: The NMDAR on striatal neurons is essential for the development of cocaine cue reactivity in the place conditioning paradigm. Our finding identifies a brain region whose constitutive NMDAR level serves as a determinant for susceptibility to this aspect of cocaine addiction.",
keywords = "Addiction, cocaine, conditional knockout mouse, conditioned place preference, cue reactivity, striatum",
author = "Soh Agatsuma and Dang, {Mai T.} and Yuqing Li and Noboru Hiroi",
year = "2010",
month = "4",
day = "15",
doi = "10.1016/j.biopsych.2009.12.023",
language = "English (US)",
volume = "67",
pages = "778--780",
journal = "Biological Psychiatry",
issn = "0006-3223",
publisher = "Elsevier USA",
number = "8",

}

TY - JOUR

T1 - N-Methyl-D-Aspartic Acid Receptors on Striatal Neurons Are Essential for Cocaine Cue Reactivity in Mice

AU - Agatsuma, Soh

AU - Dang, Mai T.

AU - Li, Yuqing

AU - Hiroi, Noboru

PY - 2010/4/15

Y1 - 2010/4/15

N2 - Background: Environmental cues associated with cocaine evoke craving and seeking. This process, termed cue reactivity, is a critical element of cocaine addiction. Although glutamatergic neurotransmission has been implicated in this effect of cocaine, the precise subtype and localization in the brain of the glutamatergic receptor critical for cocaine cue reactivity is not well-understood. Methods: We used a conditional N-methyl-D-aspartic acid receptor (NMDAR) knockout mouse whose NMDAR gene was deleted by Cre expression restricted to striatal neurons. To evaluate the role of NMDAR in cocaine cue reactivity, conditional knockout mice and control mice (n = 5-8/group) were conditioned for place preference with cocaine (5 and 10 mg/kg SC) for 3 days. Their subsequent place preference was examined in a drug-free state. Results: Although control mice developed cocaine conditioned place preference, mice deficient for NMDAR on striatal neurons failed to develop conditioned place preference. Conclusions: The NMDAR on striatal neurons is essential for the development of cocaine cue reactivity in the place conditioning paradigm. Our finding identifies a brain region whose constitutive NMDAR level serves as a determinant for susceptibility to this aspect of cocaine addiction.

AB - Background: Environmental cues associated with cocaine evoke craving and seeking. This process, termed cue reactivity, is a critical element of cocaine addiction. Although glutamatergic neurotransmission has been implicated in this effect of cocaine, the precise subtype and localization in the brain of the glutamatergic receptor critical for cocaine cue reactivity is not well-understood. Methods: We used a conditional N-methyl-D-aspartic acid receptor (NMDAR) knockout mouse whose NMDAR gene was deleted by Cre expression restricted to striatal neurons. To evaluate the role of NMDAR in cocaine cue reactivity, conditional knockout mice and control mice (n = 5-8/group) were conditioned for place preference with cocaine (5 and 10 mg/kg SC) for 3 days. Their subsequent place preference was examined in a drug-free state. Results: Although control mice developed cocaine conditioned place preference, mice deficient for NMDAR on striatal neurons failed to develop conditioned place preference. Conclusions: The NMDAR on striatal neurons is essential for the development of cocaine cue reactivity in the place conditioning paradigm. Our finding identifies a brain region whose constitutive NMDAR level serves as a determinant for susceptibility to this aspect of cocaine addiction.

KW - Addiction

KW - cocaine

KW - conditional knockout mouse

KW - conditioned place preference

KW - cue reactivity

KW - striatum

UR - http://www.scopus.com/inward/record.url?scp=77950019512&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950019512&partnerID=8YFLogxK

U2 - 10.1016/j.biopsych.2009.12.023

DO - 10.1016/j.biopsych.2009.12.023

M3 - Article

C2 - 20149346

AN - SCOPUS:77950019512

VL - 67

SP - 778

EP - 780

JO - Biological Psychiatry

JF - Biological Psychiatry

SN - 0006-3223

IS - 8

ER -