N-Acetylchitooligosaccharide is a potent angiogenic inhibitor both in vivo and in vitro

Zheng Wang; Lanhong Zheng; Shaoli Yang; Rongli Niu; Edward Chu; Xiukun Lin

doi:10.1016/j.bbrc.2007.03.094

N-Acetylchitooligosaccharide is a potent angiogenic inhibitor both in vivo and in vitro

Zheng Wang, Lanhong Zheng, Shaoli Yang, Rongli Niu, Edward Chu, Xiukun Lin

Research output: Contribution to journal › Article › peer-review

76 Scopus citations

Abstract

N-Acetylchitooligosaccharide (N-acetyl-COs) was prepared by N-acetylation of chitooligosaccharide (COs). In vitro study using human umbilical vein endothelial cells (HUVECs) revealed that both N-acetyl-COs and COs inhibited the proliferation of HUVECs by inducing apoptosis. Treatment of HUVECs by N-acetyl-COs resulted in a significant reduction of density of the migration cells and repressed tubulogenesis process. The antiangiogenic effects of the oligosaccharides were further evaluated using in vivo zebrafish angiogenesis model, and the results showed that both oligosaccharides inhibited the growth of subintestinal vessels (SIV) of zebrafish embryos in a dose-dependent manner, as observed by endogenous alkaline phosphatase (EAP) staining assay. In contrast, no cytotoxicity was found when treating the NIH3T3 and several other cancer cells with the oligosaccharides. Our results also confirmed the antiangiogenic activity of N-acetyl-COs was significantly stronger than the parent oligosaccharide, COs.

Original language	English (US)
Pages (from-to)	26-31
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	357
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2007.03.094
State	Published - May 25 2007
Externally published	Yes

Keywords

Antiangiogenesis
Chitooligosaccharide
Human umbilical vein endothelial cells
N-Acetylchitooligosaccharide
Zebrafish

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2007.03.094

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title = "N-Acetylchitooligosaccharide is a potent angiogenic inhibitor both in vivo and in vitro",

abstract = "N-Acetylchitooligosaccharide (N-acetyl-COs) was prepared by N-acetylation of chitooligosaccharide (COs). In vitro study using human umbilical vein endothelial cells (HUVECs) revealed that both N-acetyl-COs and COs inhibited the proliferation of HUVECs by inducing apoptosis. Treatment of HUVECs by N-acetyl-COs resulted in a significant reduction of density of the migration cells and repressed tubulogenesis process. The antiangiogenic effects of the oligosaccharides were further evaluated using in vivo zebrafish angiogenesis model, and the results showed that both oligosaccharides inhibited the growth of subintestinal vessels (SIV) of zebrafish embryos in a dose-dependent manner, as observed by endogenous alkaline phosphatase (EAP) staining assay. In contrast, no cytotoxicity was found when treating the NIH3T3 and several other cancer cells with the oligosaccharides. Our results also confirmed the antiangiogenic activity of N-acetyl-COs was significantly stronger than the parent oligosaccharide, COs.",

keywords = "Antiangiogenesis, Chitooligosaccharide, Human umbilical vein endothelial cells, N-Acetylchitooligosaccharide, Zebrafish",

author = "Zheng Wang and Lanhong Zheng and Shaoli Yang and Rongli Niu and Edward Chu and Xiukun Lin",

note = "Funding Information: We are grateful to Dr. Wanshun Liu (Ocean University of China, China) for the support of preparation of COs and N-acetyl-COs. Also, we thank Dr. Pui-Kai Li (The Ohio State University, USA) for generously providing us SU5416. This work is supported by National Scientific Foundation of China, No. 30472043, and the Department of Science and Technology of Shandong province, No. 2004C07.",

year = "2007",

month = may,

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doi = "10.1016/j.bbrc.2007.03.094",

language = "English (US)",

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pages = "26--31",

journal = "Biochemical and Biophysical Research Communications",

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TY - JOUR

T1 - N-Acetylchitooligosaccharide is a potent angiogenic inhibitor both in vivo and in vitro

AU - Wang, Zheng

AU - Zheng, Lanhong

AU - Yang, Shaoli

AU - Niu, Rongli

AU - Chu, Edward

AU - Lin, Xiukun

N1 - Funding Information: We are grateful to Dr. Wanshun Liu (Ocean University of China, China) for the support of preparation of COs and N-acetyl-COs. Also, we thank Dr. Pui-Kai Li (The Ohio State University, USA) for generously providing us SU5416. This work is supported by National Scientific Foundation of China, No. 30472043, and the Department of Science and Technology of Shandong province, No. 2004C07.

PY - 2007/5/25

Y1 - 2007/5/25

N2 - N-Acetylchitooligosaccharide (N-acetyl-COs) was prepared by N-acetylation of chitooligosaccharide (COs). In vitro study using human umbilical vein endothelial cells (HUVECs) revealed that both N-acetyl-COs and COs inhibited the proliferation of HUVECs by inducing apoptosis. Treatment of HUVECs by N-acetyl-COs resulted in a significant reduction of density of the migration cells and repressed tubulogenesis process. The antiangiogenic effects of the oligosaccharides were further evaluated using in vivo zebrafish angiogenesis model, and the results showed that both oligosaccharides inhibited the growth of subintestinal vessels (SIV) of zebrafish embryos in a dose-dependent manner, as observed by endogenous alkaline phosphatase (EAP) staining assay. In contrast, no cytotoxicity was found when treating the NIH3T3 and several other cancer cells with the oligosaccharides. Our results also confirmed the antiangiogenic activity of N-acetyl-COs was significantly stronger than the parent oligosaccharide, COs.

AB - N-Acetylchitooligosaccharide (N-acetyl-COs) was prepared by N-acetylation of chitooligosaccharide (COs). In vitro study using human umbilical vein endothelial cells (HUVECs) revealed that both N-acetyl-COs and COs inhibited the proliferation of HUVECs by inducing apoptosis. Treatment of HUVECs by N-acetyl-COs resulted in a significant reduction of density of the migration cells and repressed tubulogenesis process. The antiangiogenic effects of the oligosaccharides were further evaluated using in vivo zebrafish angiogenesis model, and the results showed that both oligosaccharides inhibited the growth of subintestinal vessels (SIV) of zebrafish embryos in a dose-dependent manner, as observed by endogenous alkaline phosphatase (EAP) staining assay. In contrast, no cytotoxicity was found when treating the NIH3T3 and several other cancer cells with the oligosaccharides. Our results also confirmed the antiangiogenic activity of N-acetyl-COs was significantly stronger than the parent oligosaccharide, COs.

KW - Antiangiogenesis

KW - Chitooligosaccharide

KW - Human umbilical vein endothelial cells

KW - N-Acetylchitooligosaccharide

KW - Zebrafish

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N-Acetylchitooligosaccharide is a potent angiogenic inhibitor both in vivo and in vitro

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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