N-Acetylchitooligosaccharide is a potent angiogenic inhibitor both in vivo and in vitro

Zheng Wang, Lanhong Zheng, Shaoli Yang, Rongli Niu, Edward Chu, Xiukun Lin

Research output: Contribution to journalArticlepeer-review

68 Scopus citations

Abstract

N-Acetylchitooligosaccharide (N-acetyl-COs) was prepared by N-acetylation of chitooligosaccharide (COs). In vitro study using human umbilical vein endothelial cells (HUVECs) revealed that both N-acetyl-COs and COs inhibited the proliferation of HUVECs by inducing apoptosis. Treatment of HUVECs by N-acetyl-COs resulted in a significant reduction of density of the migration cells and repressed tubulogenesis process. The antiangiogenic effects of the oligosaccharides were further evaluated using in vivo zebrafish angiogenesis model, and the results showed that both oligosaccharides inhibited the growth of subintestinal vessels (SIV) of zebrafish embryos in a dose-dependent manner, as observed by endogenous alkaline phosphatase (EAP) staining assay. In contrast, no cytotoxicity was found when treating the NIH3T3 and several other cancer cells with the oligosaccharides. Our results also confirmed the antiangiogenic activity of N-acetyl-COs was significantly stronger than the parent oligosaccharide, COs.

Original languageEnglish (US)
Pages (from-to)26-31
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume357
Issue number1
DOIs
StatePublished - May 25 2007
Externally publishedYes

Keywords

  • Antiangiogenesis
  • Chitooligosaccharide
  • Human umbilical vein endothelial cells
  • N-Acetylchitooligosaccharide
  • Zebrafish

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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