Myosin X is a downstream effector of PI(3)K during phagocytosis

Dianne Cox, Jonathan S. Berg, Michael Cammer, John O. Chinegwundoh, Benjamin M. Dale, Richard E. Cheney, Steven Greenberg

Research output: Contribution to journalArticle

160 Citations (Scopus)

Abstract

Phagocytosis is a phosphatidylinositol-3-OH-kinase (PI(3)K)-dependent process in macrophages. We identified Myo10 (Myosin-X), an unconventional myosin with pleckstrin homology (PH) domains, as a potential downstream target of PI(3)K. Myo10 was recruited to phagocytic cups in a wortmannin-sensitive manner. Expression of a truncation construct of Myo10 (Myo10 tail) in a macrophage cell line or cytosolic loading of anti-Myo10 antibodies in bovine alveolar macrophages inhibited phagocytosis. In contrast, expression of a Myo10 tail construct containing a point mutation in one of its PH domains failed to inhibit phagocytosis. Expression of Myo10 tail inhibited spreading, but not adhesion, on IgG-coated substrates, consistent with a function for Myo10 in pseudopod extension. We propose that Myo10 provides a molecular link between PI(3)K and pseudopod extension during phagocytosis.

Original languageEnglish (US)
Pages (from-to)469-477
Number of pages9
JournalNature Cell Biology
Volume4
Issue number7
DOIs
StatePublished - 2002
Externally publishedYes

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Phosphatidylinositol 3-Kinase
Myosins
Phagocytosis
Pseudopodia
Macrophages
Alveolar Macrophages
Point Mutation
Anti-Idiotypic Antibodies
Immunoglobulin G
Cell Line
hydroxide ion
Pleckstrin Homology Domains

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cox, D., Berg, J. S., Cammer, M., Chinegwundoh, J. O., Dale, B. M., Cheney, R. E., & Greenberg, S. (2002). Myosin X is a downstream effector of PI(3)K during phagocytosis. Nature Cell Biology, 4(7), 469-477. https://doi.org/10.1038/ncb805

Myosin X is a downstream effector of PI(3)K during phagocytosis. / Cox, Dianne; Berg, Jonathan S.; Cammer, Michael; Chinegwundoh, John O.; Dale, Benjamin M.; Cheney, Richard E.; Greenberg, Steven.

In: Nature Cell Biology, Vol. 4, No. 7, 2002, p. 469-477.

Research output: Contribution to journalArticle

Cox, D, Berg, JS, Cammer, M, Chinegwundoh, JO, Dale, BM, Cheney, RE & Greenberg, S 2002, 'Myosin X is a downstream effector of PI(3)K during phagocytosis', Nature Cell Biology, vol. 4, no. 7, pp. 469-477. https://doi.org/10.1038/ncb805
Cox D, Berg JS, Cammer M, Chinegwundoh JO, Dale BM, Cheney RE et al. Myosin X is a downstream effector of PI(3)K during phagocytosis. Nature Cell Biology. 2002;4(7):469-477. https://doi.org/10.1038/ncb805
Cox, Dianne ; Berg, Jonathan S. ; Cammer, Michael ; Chinegwundoh, John O. ; Dale, Benjamin M. ; Cheney, Richard E. ; Greenberg, Steven. / Myosin X is a downstream effector of PI(3)K during phagocytosis. In: Nature Cell Biology. 2002 ; Vol. 4, No. 7. pp. 469-477.
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