Myosin phenotype and bioenergetic characteristics of rat respiratory muscles

Scott K. Powers, Haydar A. Demirel, Jeff S. Coombes, Louise Fletcher, Corinne Calliaud, Iannis Vrabas, David J. Prezant

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

These experiments examined the myosin phenotype and bioenergetic enzyme activities in rat respiratory muscles. Muscle samples were removed from adult female Sprague-Dawley rats (N = 8) and analyzed to determine the myosin heavy chain (MHC) and light chain (MLC) isoform content as well as the activities of myofibrillar ATPase (mATPase), citrate synthase (CS; Krebs cycle enzyme), and lactate dehydrogenase (LDH; glycolytic enzyme). Analysis revealed that CS activity and the % type I MHC and %IId MHC isoforms were greater in the costal diaphragm (CO-D) compared with those in the crural diaphragm (CR-D). In contrast, the % type IIb MHC was higher in the CR-D compared with that in the CO-D. LDH and mATPase activity were lower in both the CO-D and CR-D compared with that in the parasternal intercostals (PI), external intercostals (EI), internal intercostals (II), rectus abdominis (RA), and sternomastoid (SM) muscles. CS activity, % type I MHC, %IIa MHC, and the ratio of slow to total alkali MLC (1s/1s + 1f + 3f) were greater in the CO-D and CR-D compared with those in all other respiratory muscles. The RA contained the highest (P < 0.05) % type IIb MHC and lowest CS activity compared with that in all other muscles. Finally, CS activity, mATPase activity, and MHC phenotype did not differ among the PI, EI, II, and SM muscles. These differences in biochemical properties provide the muscles of the respiratory pump with great versatility in functional properties.

Original languageEnglish (US)
Pages (from-to)1573-1579
Number of pages7
JournalMedicine and Science in Sports and Exercise
Volume29
Issue number12
DOIs
StatePublished - 1997
Externally publishedYes

Fingerprint

Respiratory Muscles
Myosin Heavy Chains
Myosins
Energy Metabolism
Diaphragm
Phenotype
Leg
Adenosine Triphosphatases
Rectus Abdominis
Muscles
Protein Isoforms
Enzymes
Intercostal Muscles
Light
Citrate (si)-Synthase
Citric Acid Cycle
Alkalies
Carbon Monoxide
L-Lactate Dehydrogenase
Sprague Dawley Rats

Keywords

  • DIAPHRAGM
  • GLYCOLYTIC CAPACITY
  • LOCOMOTOR MUSCLES
  • OXIDATIVE CAPACITY
  • VENTILATION

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Physical Therapy, Sports Therapy and Rehabilitation
  • Orthopedics and Sports Medicine

Cite this

Myosin phenotype and bioenergetic characteristics of rat respiratory muscles. / Powers, Scott K.; Demirel, Haydar A.; Coombes, Jeff S.; Fletcher, Louise; Calliaud, Corinne; Vrabas, Iannis; Prezant, David J.

In: Medicine and Science in Sports and Exercise, Vol. 29, No. 12, 1997, p. 1573-1579.

Research output: Contribution to journalArticle

Powers, Scott K. ; Demirel, Haydar A. ; Coombes, Jeff S. ; Fletcher, Louise ; Calliaud, Corinne ; Vrabas, Iannis ; Prezant, David J. / Myosin phenotype and bioenergetic characteristics of rat respiratory muscles. In: Medicine and Science in Sports and Exercise. 1997 ; Vol. 29, No. 12. pp. 1573-1579.
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AU - Demirel, Haydar A.

AU - Coombes, Jeff S.

AU - Fletcher, Louise

AU - Calliaud, Corinne

AU - Vrabas, Iannis

AU - Prezant, David J.

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AB - These experiments examined the myosin phenotype and bioenergetic enzyme activities in rat respiratory muscles. Muscle samples were removed from adult female Sprague-Dawley rats (N = 8) and analyzed to determine the myosin heavy chain (MHC) and light chain (MLC) isoform content as well as the activities of myofibrillar ATPase (mATPase), citrate synthase (CS; Krebs cycle enzyme), and lactate dehydrogenase (LDH; glycolytic enzyme). Analysis revealed that CS activity and the % type I MHC and %IId MHC isoforms were greater in the costal diaphragm (CO-D) compared with those in the crural diaphragm (CR-D). In contrast, the % type IIb MHC was higher in the CR-D compared with that in the CO-D. LDH and mATPase activity were lower in both the CO-D and CR-D compared with that in the parasternal intercostals (PI), external intercostals (EI), internal intercostals (II), rectus abdominis (RA), and sternomastoid (SM) muscles. CS activity, % type I MHC, %IIa MHC, and the ratio of slow to total alkali MLC (1s/1s + 1f + 3f) were greater in the CO-D and CR-D compared with those in all other respiratory muscles. The RA contained the highest (P < 0.05) % type IIb MHC and lowest CS activity compared with that in all other muscles. Finally, CS activity, mATPase activity, and MHC phenotype did not differ among the PI, EI, II, and SM muscles. These differences in biochemical properties provide the muscles of the respiratory pump with great versatility in functional properties.

KW - DIAPHRAGM

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KW - OXIDATIVE CAPACITY

KW - VENTILATION

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