Abstract
Nonmuscle myosin-IIA (NMHC-IIA) heavy chain phosphorylation has gained recognition as an important feature of myosin-II regulation. In previous work, we showed that phosphorylation on S1943 promotes myosin-IIA filament disassembly in vitro and enhances EGF-stimulated lamellipod extension of breast tumor cells. However, the contribution of NMHC-IIA S1943 phosphorylation to the modulation of invasive cellular behavior and metastasis has not been examined. Stable expression of phosphomimetic (S1943E) or non-phosphorylatable (S1943A) NMHC-IIA in breast cancer cells revealed that S1943 phosphorylation enhances invadopodia function, and is critical for matrix degradation in vitro and experimental metastasis in vivo. These studies demonstrate a novel link between NMHC-IIA S1943 phosphorylation, the regulation of extracellular matrix degradation and tumor cell invasion and metastasis.
Original language | English (US) |
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Pages (from-to) | 273-282 |
Number of pages | 10 |
Journal | Experimental Cell Research |
Volume | 370 |
Issue number | 2 |
DOIs | |
State | Published - Sep 15 2018 |
Keywords
- Invadopodia
- Invasion
- Matrix degradation
- Myosin-II
- Phosphorylation
ASJC Scopus subject areas
- Cell Biology