Myo2p, a class V myosin in budding yeast, associates with a large ribonucleic acid-protein complex that contains mRNAs and subunits of the RNA-processing body

Wakam Chang, Rania F. Zaarour, Samara Reck-Peterson, John Rinn, Robert H. Singer, Michael Snyder, Peter Novick, Mark S. Mooseker

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Myo2p is an essential class V myosin in budding yeast with several identified functions in organelle trafficking and spindle orientation. The present study demonstrates that Myo2p is a component of a large RNA-containing complex (Myo2p-RNP) that is distinct from polysomes based on sedimentation analysis and lack of ribosomal subunits in the Myo2p-RNP. Microarray analysis of RNAs that coimmunoprecipitate with Myo2p revealed the presence of a large number of mRNAs in this complex. The Myo2p-RNA complex is in part composed of the RNA processing body (P-body) based on coprecipitation with P-body protein subunits and partial colocalization of Myo2p with P-bodies. P-body disassembly is delayed in the motor mutant, myo2-66, indicating that Myo2p may facilitate the release of mRNAs from the P-body. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)491-502
Number of pages12
JournalRNA
Volume14
Issue number3
DOIs
StatePublished - Mar 2008

Fingerprint

Myosin Type V
Saccharomycetales
RNA
Messenger RNA
Proteins
Ribosome Subunits
Polyribosomes
Protein Subunits
Microarray Analysis
Organelles

Keywords

  • Myosin V
  • P-body
  • RNP
  • Saccharomyces cerevisiae
  • Unconventional myosin

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Medicine(all)

Cite this

Myo2p, a class V myosin in budding yeast, associates with a large ribonucleic acid-protein complex that contains mRNAs and subunits of the RNA-processing body. / Chang, Wakam; Zaarour, Rania F.; Reck-Peterson, Samara; Rinn, John; Singer, Robert H.; Snyder, Michael; Novick, Peter; Mooseker, Mark S.

In: RNA, Vol. 14, No. 3, 03.2008, p. 491-502.

Research output: Contribution to journalArticle

Chang, Wakam ; Zaarour, Rania F. ; Reck-Peterson, Samara ; Rinn, John ; Singer, Robert H. ; Snyder, Michael ; Novick, Peter ; Mooseker, Mark S. / Myo2p, a class V myosin in budding yeast, associates with a large ribonucleic acid-protein complex that contains mRNAs and subunits of the RNA-processing body. In: RNA. 2008 ; Vol. 14, No. 3. pp. 491-502.
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AU - Zaarour, Rania F.

AU - Reck-Peterson, Samara

AU - Rinn, John

AU - Singer, Robert H.

AU - Snyder, Michael

AU - Novick, Peter

AU - Mooseker, Mark S.

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AB - Myo2p is an essential class V myosin in budding yeast with several identified functions in organelle trafficking and spindle orientation. The present study demonstrates that Myo2p is a component of a large RNA-containing complex (Myo2p-RNP) that is distinct from polysomes based on sedimentation analysis and lack of ribosomal subunits in the Myo2p-RNP. Microarray analysis of RNAs that coimmunoprecipitate with Myo2p revealed the presence of a large number of mRNAs in this complex. The Myo2p-RNA complex is in part composed of the RNA processing body (P-body) based on coprecipitation with P-body protein subunits and partial colocalization of Myo2p with P-bodies. P-body disassembly is delayed in the motor mutant, myo2-66, indicating that Myo2p may facilitate the release of mRNAs from the P-body. Published by Cold Spring Harbor Laboratory Press.

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