Mycobacterium tuberculosis WhiB3 interacts with RpoV to affect host survival but is dispensable for in vivo growth

Adrie J.C. Steyn, Desmond M. Collins, Mary K. Hondalus, William R. Jacobs, R. Pamela Kawakami, Barry R. Bloom

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Abstract

Previous work established that the principal sigma factor (RpoV) of virulent Mycobacterium bovis, a member of the Mycobacterium tuberculosis complex, restores virulence to an attenuated strain containing a point mutation (Arg-515→His) in the 4.2 domain of RpoV. We used the 4.2 domain of RpoV as bait in a yeast two-hybrid screen of an M. tuberculosis H37Rv library and identified a putative transcription factor, WhiB3, which selectively interacts with the 4.2 domain of RpoV in virulent strains but not with the mutated (Arg-515→His) allele. Infection of mice and guinea pigs with a M. tuberculosis H37Rv whiB3 deletion mutant strain showed that whiB3 is not necessary for in vivo bacterial replication in either animal model. In contrast, an M. bovis whiB3 deletion mutant was completely attenuated for growth in guinea pigs. However, we found that immunocompetent mice infected with the M. tuberculosis H37Rv whiB3 mutant strain had significantly longer mean survival times as compared with mice challenged with wild-type M. tuberculosis. Remarkably, the bacterial organ burdens of both mutant and wild-type infected mice were identical during the acute and persistent phases of infection. Our results imply that M. tuberculosis replication per se is not a sufficient condition for virulence in vivo. They also indicate a different role for M. bovis and M. tuberculosis whiB3 genes in pathogenesis generated in different animal models. We propose that M. tuberculosis WhiB3 functions as a transcription factor regulating genes that influence the immune response of the host.

Original languageEnglish (US)
Pages (from-to)3147-3152
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number5
DOIs
StatePublished - Mar 5 2002

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Mycobacterium tuberculosis
Growth
Mycobacterium bovis
Virulence
Guinea Pigs
Transcription Factors
Animal Models
Sigma Factor
Infection
Point Mutation
Genes
Libraries
Yeasts
Alleles

ASJC Scopus subject areas

  • General

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Mycobacterium tuberculosis WhiB3 interacts with RpoV to affect host survival but is dispensable for in vivo growth. / Steyn, Adrie J.C.; Collins, Desmond M.; Hondalus, Mary K.; Jacobs, William R.; Pamela Kawakami, R.; Bloom, Barry R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 5, 05.03.2002, p. 3147-3152.

Research output: Contribution to journalArticle

Steyn, Adrie J.C. ; Collins, Desmond M. ; Hondalus, Mary K. ; Jacobs, William R. ; Pamela Kawakami, R. ; Bloom, Barry R. / Mycobacterium tuberculosis WhiB3 interacts with RpoV to affect host survival but is dispensable for in vivo growth. In: Proceedings of the National Academy of Sciences of the United States of America. 2002 ; Vol. 99, No. 5. pp. 3147-3152.
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