Mycobacterium tuberculosis universal stress protein Rv2623 interacts with the putative ATP binding cassette (APC) transporter Rv1747 to regulate mycobacterial growth

Lisa N. Glass, Ganduri Swapna, Sivagami Sundaram Chavadi, Jo Ann M. Tufariello, Kaixia Mi, Joshua E. Drumm, Tu Kiet T. Lam, Guofeng Zhu, Chenyang Zhan, Catherine Vilchéze, Jesus Arcos, Yong Chen, Lijun Bi, Simren Mehta, Steven A. Porcelli, Steven C. Almo, Syun-Ru Yeh, William R. Jacobs, Jordi B. Torrelles, John Chan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have previously shown that the Mycobacterium tuberculosis universal stress protein Rv2623 regulates mycobacterial growth and may be required for the establishment of tuberculous persistence. Here, yeast two-hybrid and affinity chromatography experiments have demonstrated that Rv2623 interacts with one of the two forkhead-associated domains (FHA I) of Rv1747, a putative ATP-binding cassette transporter annotated to export lipooligosaccharides. FHA domains are signaling protein modules that mediate protein-protein interactions to modulate a wide variety of biological processes via binding to conserved phosphorylated threonine (pT)-containing oligopeptides of the interactors. Biochemical, immunochemical and mass spectrometric studies have shown that Rv2623 harbors pT and specifically identified threonine 237 as a phosphorylated residue. Relative to wild-type Rv2623 (Rv2623WT), a mutant protein in which T237 has been replaced with a non-phosphorylatable alanine (Rv2623T237A) exhibits decreased interaction with the Rv1747 FHA I domain and diminished growth-regulatory capacity. Interestingly, compared to WT bacilli, an M. tuberculosis Rv2623 null mutant (ΔRv2623) displays enhanced expression of phosphatidyl-myo-inositol mannosides (PIMs), while the ΔRv1747 mutant expresses decreased levels of PIMs. Animal studies have previously shown that ΔRv2623 is hypervirulent, while ΔRv1747 is growth-attenuated. Collectively, these data have provided evidence that Rv2623 interacts with Rv1747 to regulate mycobacterial growth; and this interaction is mediated via the recognition of the conserved Rv2623 pT237-containing FHA-binding motif by the Rv1747 FHA I domain. The divergent aberrant PIM profiles and the opposing in vivo growth phenotypes of ΔRv2623 and ΔRv1747, together with the annotated lipooligosaccharide exporter function of Rv1747, suggest that Rv2623 interacts with Rv1747 to modulate mycobacterial growth by negatively regulating the activity of Rv1747; and that Rv1747 might function as a transporter of PIMs. Because these glycolipids are major mycobacterial cell envelope components that can impact on the immune response, our findings raise the possibility that Rv2623 may regulate bacterial growth, virulence, and entry into persistence, at least in part, by modulating the levels of bacillary PIM expression, perhaps through negatively regulating the Rv1747-dependent export of the immunomodulatory PIMs to alter host-pathogen interaction, thereby influencing the fate of M. tuberculosis in vivo.

Original languageEnglish (US)
Article numbere1006515
JournalPLoS Pathogens
Volume13
Issue number7
DOIs
StatePublished - Jul 1 2017

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ATP-Binding Cassette Transporters
Heat-Shock Proteins
Mycobacterium tuberculosis
Growth
Threonine
Biological Phenomena
Host-Pathogen Interactions
Oligopeptides
Glycolipids
Mutant Proteins
Cellular Structures
Affinity Chromatography
Alanine
Bacillus
Virulence
phosphatidylinositol mannoside
Proteins
Yeasts
Phenotype

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

Cite this

Mycobacterium tuberculosis universal stress protein Rv2623 interacts with the putative ATP binding cassette (APC) transporter Rv1747 to regulate mycobacterial growth. / Glass, Lisa N.; Swapna, Ganduri; Chavadi, Sivagami Sundaram; Tufariello, Jo Ann M.; Mi, Kaixia; Drumm, Joshua E.; Lam, Tu Kiet T.; Zhu, Guofeng; Zhan, Chenyang; Vilchéze, Catherine; Arcos, Jesus; Chen, Yong; Bi, Lijun; Mehta, Simren; Porcelli, Steven A.; Almo, Steven C.; Yeh, Syun-Ru; Jacobs, William R.; Torrelles, Jordi B.; Chan, John.

In: PLoS Pathogens, Vol. 13, No. 7, e1006515, 01.07.2017.

Research output: Contribution to journalArticle

Glass, LN, Swapna, G, Chavadi, SS, Tufariello, JAM, Mi, K, Drumm, JE, Lam, TKT, Zhu, G, Zhan, C, Vilchéze, C, Arcos, J, Chen, Y, Bi, L, Mehta, S, Porcelli, SA, Almo, SC, Yeh, S-R, Jacobs, WR, Torrelles, JB & Chan, J 2017, 'Mycobacterium tuberculosis universal stress protein Rv2623 interacts with the putative ATP binding cassette (APC) transporter Rv1747 to regulate mycobacterial growth', PLoS Pathogens, vol. 13, no. 7, e1006515. https://doi.org/10.1371/journal.ppat.1006515
Glass, Lisa N. ; Swapna, Ganduri ; Chavadi, Sivagami Sundaram ; Tufariello, Jo Ann M. ; Mi, Kaixia ; Drumm, Joshua E. ; Lam, Tu Kiet T. ; Zhu, Guofeng ; Zhan, Chenyang ; Vilchéze, Catherine ; Arcos, Jesus ; Chen, Yong ; Bi, Lijun ; Mehta, Simren ; Porcelli, Steven A. ; Almo, Steven C. ; Yeh, Syun-Ru ; Jacobs, William R. ; Torrelles, Jordi B. ; Chan, John. / Mycobacterium tuberculosis universal stress protein Rv2623 interacts with the putative ATP binding cassette (APC) transporter Rv1747 to regulate mycobacterial growth. In: PLoS Pathogens. 2017 ; Vol. 13, No. 7.
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abstract = "We have previously shown that the Mycobacterium tuberculosis universal stress protein Rv2623 regulates mycobacterial growth and may be required for the establishment of tuberculous persistence. Here, yeast two-hybrid and affinity chromatography experiments have demonstrated that Rv2623 interacts with one of the two forkhead-associated domains (FHA I) of Rv1747, a putative ATP-binding cassette transporter annotated to export lipooligosaccharides. FHA domains are signaling protein modules that mediate protein-protein interactions to modulate a wide variety of biological processes via binding to conserved phosphorylated threonine (pT)-containing oligopeptides of the interactors. Biochemical, immunochemical and mass spectrometric studies have shown that Rv2623 harbors pT and specifically identified threonine 237 as a phosphorylated residue. Relative to wild-type Rv2623 (Rv2623WT), a mutant protein in which T237 has been replaced with a non-phosphorylatable alanine (Rv2623T237A) exhibits decreased interaction with the Rv1747 FHA I domain and diminished growth-regulatory capacity. Interestingly, compared to WT bacilli, an M. tuberculosis Rv2623 null mutant (ΔRv2623) displays enhanced expression of phosphatidyl-myo-inositol mannosides (PIMs), while the ΔRv1747 mutant expresses decreased levels of PIMs. Animal studies have previously shown that ΔRv2623 is hypervirulent, while ΔRv1747 is growth-attenuated. Collectively, these data have provided evidence that Rv2623 interacts with Rv1747 to regulate mycobacterial growth; and this interaction is mediated via the recognition of the conserved Rv2623 pT237-containing FHA-binding motif by the Rv1747 FHA I domain. The divergent aberrant PIM profiles and the opposing in vivo growth phenotypes of ΔRv2623 and ΔRv1747, together with the annotated lipooligosaccharide exporter function of Rv1747, suggest that Rv2623 interacts with Rv1747 to modulate mycobacterial growth by negatively regulating the activity of Rv1747; and that Rv1747 might function as a transporter of PIMs. Because these glycolipids are major mycobacterial cell envelope components that can impact on the immune response, our findings raise the possibility that Rv2623 may regulate bacterial growth, virulence, and entry into persistence, at least in part, by modulating the levels of bacillary PIM expression, perhaps through negatively regulating the Rv1747-dependent export of the immunomodulatory PIMs to alter host-pathogen interaction, thereby influencing the fate of M. tuberculosis in vivo.",
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T1 - Mycobacterium tuberculosis universal stress protein Rv2623 interacts with the putative ATP binding cassette (APC) transporter Rv1747 to regulate mycobacterial growth

AU - Glass, Lisa N.

AU - Swapna, Ganduri

AU - Chavadi, Sivagami Sundaram

AU - Tufariello, Jo Ann M.

AU - Mi, Kaixia

AU - Drumm, Joshua E.

AU - Lam, Tu Kiet T.

AU - Zhu, Guofeng

AU - Zhan, Chenyang

AU - Vilchéze, Catherine

AU - Arcos, Jesus

AU - Chen, Yong

AU - Bi, Lijun

AU - Mehta, Simren

AU - Porcelli, Steven A.

AU - Almo, Steven C.

AU - Yeh, Syun-Ru

AU - Jacobs, William R.

AU - Torrelles, Jordi B.

AU - Chan, John

PY - 2017/7/1

Y1 - 2017/7/1

N2 - We have previously shown that the Mycobacterium tuberculosis universal stress protein Rv2623 regulates mycobacterial growth and may be required for the establishment of tuberculous persistence. Here, yeast two-hybrid and affinity chromatography experiments have demonstrated that Rv2623 interacts with one of the two forkhead-associated domains (FHA I) of Rv1747, a putative ATP-binding cassette transporter annotated to export lipooligosaccharides. FHA domains are signaling protein modules that mediate protein-protein interactions to modulate a wide variety of biological processes via binding to conserved phosphorylated threonine (pT)-containing oligopeptides of the interactors. Biochemical, immunochemical and mass spectrometric studies have shown that Rv2623 harbors pT and specifically identified threonine 237 as a phosphorylated residue. Relative to wild-type Rv2623 (Rv2623WT), a mutant protein in which T237 has been replaced with a non-phosphorylatable alanine (Rv2623T237A) exhibits decreased interaction with the Rv1747 FHA I domain and diminished growth-regulatory capacity. Interestingly, compared to WT bacilli, an M. tuberculosis Rv2623 null mutant (ΔRv2623) displays enhanced expression of phosphatidyl-myo-inositol mannosides (PIMs), while the ΔRv1747 mutant expresses decreased levels of PIMs. Animal studies have previously shown that ΔRv2623 is hypervirulent, while ΔRv1747 is growth-attenuated. Collectively, these data have provided evidence that Rv2623 interacts with Rv1747 to regulate mycobacterial growth; and this interaction is mediated via the recognition of the conserved Rv2623 pT237-containing FHA-binding motif by the Rv1747 FHA I domain. The divergent aberrant PIM profiles and the opposing in vivo growth phenotypes of ΔRv2623 and ΔRv1747, together with the annotated lipooligosaccharide exporter function of Rv1747, suggest that Rv2623 interacts with Rv1747 to modulate mycobacterial growth by negatively regulating the activity of Rv1747; and that Rv1747 might function as a transporter of PIMs. Because these glycolipids are major mycobacterial cell envelope components that can impact on the immune response, our findings raise the possibility that Rv2623 may regulate bacterial growth, virulence, and entry into persistence, at least in part, by modulating the levels of bacillary PIM expression, perhaps through negatively regulating the Rv1747-dependent export of the immunomodulatory PIMs to alter host-pathogen interaction, thereby influencing the fate of M. tuberculosis in vivo.

AB - We have previously shown that the Mycobacterium tuberculosis universal stress protein Rv2623 regulates mycobacterial growth and may be required for the establishment of tuberculous persistence. Here, yeast two-hybrid and affinity chromatography experiments have demonstrated that Rv2623 interacts with one of the two forkhead-associated domains (FHA I) of Rv1747, a putative ATP-binding cassette transporter annotated to export lipooligosaccharides. FHA domains are signaling protein modules that mediate protein-protein interactions to modulate a wide variety of biological processes via binding to conserved phosphorylated threonine (pT)-containing oligopeptides of the interactors. Biochemical, immunochemical and mass spectrometric studies have shown that Rv2623 harbors pT and specifically identified threonine 237 as a phosphorylated residue. Relative to wild-type Rv2623 (Rv2623WT), a mutant protein in which T237 has been replaced with a non-phosphorylatable alanine (Rv2623T237A) exhibits decreased interaction with the Rv1747 FHA I domain and diminished growth-regulatory capacity. Interestingly, compared to WT bacilli, an M. tuberculosis Rv2623 null mutant (ΔRv2623) displays enhanced expression of phosphatidyl-myo-inositol mannosides (PIMs), while the ΔRv1747 mutant expresses decreased levels of PIMs. Animal studies have previously shown that ΔRv2623 is hypervirulent, while ΔRv1747 is growth-attenuated. Collectively, these data have provided evidence that Rv2623 interacts with Rv1747 to regulate mycobacterial growth; and this interaction is mediated via the recognition of the conserved Rv2623 pT237-containing FHA-binding motif by the Rv1747 FHA I domain. The divergent aberrant PIM profiles and the opposing in vivo growth phenotypes of ΔRv2623 and ΔRv1747, together with the annotated lipooligosaccharide exporter function of Rv1747, suggest that Rv2623 interacts with Rv1747 to modulate mycobacterial growth by negatively regulating the activity of Rv1747; and that Rv1747 might function as a transporter of PIMs. Because these glycolipids are major mycobacterial cell envelope components that can impact on the immune response, our findings raise the possibility that Rv2623 may regulate bacterial growth, virulence, and entry into persistence, at least in part, by modulating the levels of bacillary PIM expression, perhaps through negatively regulating the Rv1747-dependent export of the immunomodulatory PIMs to alter host-pathogen interaction, thereby influencing the fate of M. tuberculosis in vivo.

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