Mycobacterium smegmatis biofilm formation and sliding motility are affected by the serine/threonine protein kinase PknF

Radha Gopalaswamy, Sujatha Narayanan, William R. Jacobs, Yossef Av-Gay

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Eighteen 'eukaryotic-like' serine/threonine kinases are present in the Mycobacterium smegmatis genome. One of them encoded by the ORF 3677 demonstrates high similarity to the Mycobacterium tuberculosis protein kinase PknF. A merodiploid strain was generated, which showed reduced growth associated with irregular cell structure. The merodiploid strain displayed altered colony morphology, defective sliding motility and biofilm formation. These data indicate a role for PknF in biofilm formation, possibly associated with alterations in glycopeptidolipid composition.

Original languageEnglish (US)
Pages (from-to)121-127
Number of pages7
JournalFEMS Microbiology Letters
Volume278
Issue number1
DOIs
StatePublished - Jan 2008

Keywords

  • Biofilm
  • Cell morphology
  • GPLs
  • Mycobacteria
  • Serine/threonine kinase
  • Sliding motility

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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