Mycobacterium smegmatis biofilm formation and sliding motility are affected by the serine/threonine protein kinase PknF

Radha Gopalaswamy, Sujatha Narayanan, William R. Jacobs, Yossef Av-Gay

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Eighteen 'eukaryotic-like' serine/threonine kinases are present in the Mycobacterium smegmatis genome. One of them encoded by the ORF 3677 demonstrates high similarity to the Mycobacterium tuberculosis protein kinase PknF. A merodiploid strain was generated, which showed reduced growth associated with irregular cell structure. The merodiploid strain displayed altered colony morphology, defective sliding motility and biofilm formation. These data indicate a role for PknF in biofilm formation, possibly associated with alterations in glycopeptidolipid composition.

Original languageEnglish (US)
Pages (from-to)121-127
Number of pages7
JournalFEMS Microbiology Letters
Volume278
Issue number1
DOIs
StatePublished - Jan 1 2008

Fingerprint

Mycobacterium smegmatis
Protein-Serine-Threonine Kinases
Biofilms
Mycobacterium tuberculosis
Protein Kinases
Open Reading Frames
Genome
Growth

Keywords

  • Biofilm
  • Cell morphology
  • GPLs
  • Mycobacteria
  • Serine/threonine kinase
  • Sliding motility

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Mycobacterium smegmatis biofilm formation and sliding motility are affected by the serine/threonine protein kinase PknF. / Gopalaswamy, Radha; Narayanan, Sujatha; Jacobs, William R.; Av-Gay, Yossef.

In: FEMS Microbiology Letters, Vol. 278, No. 1, 01.01.2008, p. 121-127.

Research output: Contribution to journalArticle

@article{4d4f8a9535e54bf0811a8bc915610b86,
title = "Mycobacterium smegmatis biofilm formation and sliding motility are affected by the serine/threonine protein kinase PknF",
abstract = "Eighteen 'eukaryotic-like' serine/threonine kinases are present in the Mycobacterium smegmatis genome. One of them encoded by the ORF 3677 demonstrates high similarity to the Mycobacterium tuberculosis protein kinase PknF. A merodiploid strain was generated, which showed reduced growth associated with irregular cell structure. The merodiploid strain displayed altered colony morphology, defective sliding motility and biofilm formation. These data indicate a role for PknF in biofilm formation, possibly associated with alterations in glycopeptidolipid composition.",
keywords = "Biofilm, Cell morphology, GPLs, Mycobacteria, Serine/threonine kinase, Sliding motility",
author = "Radha Gopalaswamy and Sujatha Narayanan and Jacobs, {William R.} and Yossef Av-Gay",
year = "2008",
month = "1",
day = "1",
doi = "10.1111/j.1574-6968.2007.00989.x",
language = "English (US)",
volume = "278",
pages = "121--127",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Mycobacterium smegmatis biofilm formation and sliding motility are affected by the serine/threonine protein kinase PknF

AU - Gopalaswamy, Radha

AU - Narayanan, Sujatha

AU - Jacobs, William R.

AU - Av-Gay, Yossef

PY - 2008/1/1

Y1 - 2008/1/1

N2 - Eighteen 'eukaryotic-like' serine/threonine kinases are present in the Mycobacterium smegmatis genome. One of them encoded by the ORF 3677 demonstrates high similarity to the Mycobacterium tuberculosis protein kinase PknF. A merodiploid strain was generated, which showed reduced growth associated with irregular cell structure. The merodiploid strain displayed altered colony morphology, defective sliding motility and biofilm formation. These data indicate a role for PknF in biofilm formation, possibly associated with alterations in glycopeptidolipid composition.

AB - Eighteen 'eukaryotic-like' serine/threonine kinases are present in the Mycobacterium smegmatis genome. One of them encoded by the ORF 3677 demonstrates high similarity to the Mycobacterium tuberculosis protein kinase PknF. A merodiploid strain was generated, which showed reduced growth associated with irregular cell structure. The merodiploid strain displayed altered colony morphology, defective sliding motility and biofilm formation. These data indicate a role for PknF in biofilm formation, possibly associated with alterations in glycopeptidolipid composition.

KW - Biofilm

KW - Cell morphology

KW - GPLs

KW - Mycobacteria

KW - Serine/threonine kinase

KW - Sliding motility

UR - http://www.scopus.com/inward/record.url?scp=36749061398&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36749061398&partnerID=8YFLogxK

U2 - 10.1111/j.1574-6968.2007.00989.x

DO - 10.1111/j.1574-6968.2007.00989.x

M3 - Article

C2 - 18031532

AN - SCOPUS:36749061398

VL - 278

SP - 121

EP - 127

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 1

ER -