TY - JOUR
T1 - MxB is not responsible for the blocking of HIV-1 infection observed in alpha interferontreated cells
AU - Opp, Silvana
AU - Vieira, Daniel A.S.A.
AU - Schulte, Bianca
AU - Chanda, Sumit K.
AU - Diaz-Griffero, Felipe
N1 - Publisher Copyright:
© 2016, American Society for Microbiology.
PY - 2016
Y1 - 2016
N2 - MxB restricts HIV-1 infection by directly interacting with the HIV-1 core, which is made of viral capsid; however, the contribution of MxB to the HIV-1 restriction observed in alpha interferon (IFN-α)-treated human cells is unknown. To understand this contribution, we used HIV-1 bearing the G208R capsid mutant (HIV-1-G208R), which overcomes the restriction imposed by cells expressing MxB. Here we showed that the reason why MxB does not block HIV-1-G208R is that MxB does not interact with HIV-1 cores bearing the mutation G208R. To understand whether MxB contributes to the HIV-1 restriction imposed by IFN-α- treated human cells, we challenged IFN-α-treated cells with HIV-G208R and found that MxB does not contribute to the restriction imposed by IFN-α-treated cells. To more directly test the contribution of MxB, we challenged IFN-α-treated human cells that are knocked out for the expression of MxB with HIV-1. These experiments suggested that MxB does not contribute to the HIV-1 restriction observed in IFN-α-treated human cells.
AB - MxB restricts HIV-1 infection by directly interacting with the HIV-1 core, which is made of viral capsid; however, the contribution of MxB to the HIV-1 restriction observed in alpha interferon (IFN-α)-treated human cells is unknown. To understand this contribution, we used HIV-1 bearing the G208R capsid mutant (HIV-1-G208R), which overcomes the restriction imposed by cells expressing MxB. Here we showed that the reason why MxB does not block HIV-1-G208R is that MxB does not interact with HIV-1 cores bearing the mutation G208R. To understand whether MxB contributes to the HIV-1 restriction imposed by IFN-α- treated human cells, we challenged IFN-α-treated cells with HIV-G208R and found that MxB does not contribute to the restriction imposed by IFN-α-treated cells. To more directly test the contribution of MxB, we challenged IFN-α-treated human cells that are knocked out for the expression of MxB with HIV-1. These experiments suggested that MxB does not contribute to the HIV-1 restriction observed in IFN-α-treated human cells.
UR - http://www.scopus.com/inward/record.url?scp=84961196397&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84961196397&partnerID=8YFLogxK
U2 - 10.1128/JVI.03146-15
DO - 10.1128/JVI.03146-15
M3 - Article
C2 - 26719253
AN - SCOPUS:84961196397
SN - 0022-538X
VL - 90
SP - 3056
EP - 3064
JO - Journal of virology
JF - Journal of virology
IS - 6
ER -