MxB is not responsible for the blocking of HIV-1 infection observed in alpha interferontreated cells

Silvana Opp, Daniel A.S.A. Vieira, Bianca Schulte, Sumit K. Chanda, Felipe Diaz-Griffero

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

MxB restricts HIV-1 infection by directly interacting with the HIV-1 core, which is made of viral capsid; however, the contribution of MxB to the HIV-1 restriction observed in alpha interferon (IFN-α)-treated human cells is unknown. To understand this contribution, we used HIV-1 bearing the G208R capsid mutant (HIV-1-G208R), which overcomes the restriction imposed by cells expressing MxB. Here we showed that the reason why MxB does not block HIV-1-G208R is that MxB does not interact with HIV-1 cores bearing the mutation G208R. To understand whether MxB contributes to the HIV-1 restriction imposed by IFN-α- treated human cells, we challenged IFN-α-treated cells with HIV-G208R and found that MxB does not contribute to the restriction imposed by IFN-α-treated cells. To more directly test the contribution of MxB, we challenged IFN-α-treated human cells that are knocked out for the expression of MxB with HIV-1. These experiments suggested that MxB does not contribute to the HIV-1 restriction observed in IFN-α-treated human cells.

Original languageEnglish (US)
Pages (from-to)3056-3064
Number of pages9
JournalJournal of virology
Volume90
Issue number6
DOIs
StatePublished - Jan 1 2016

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ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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