MxB is not responsible for the blocking of HIV-1 infection observed in alpha interferontreated cells

Silvana Opp, Daniel A S A Vieira, Bianca Schulte, Sumit K. Chanda, Felipe Diaz-Griffero

Research output: Contribution to journalArticle

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Abstract

MxB restricts HIV-1 infection by directly interacting with the HIV-1 core, which is made of viral capsid; however, the contribution of MxB to the HIV-1 restriction observed in alpha interferon (IFN-α)-treated human cells is unknown. To understand this contribution, we used HIV-1 bearing the G208R capsid mutant (HIV-1-G208R), which overcomes the restriction imposed by cells expressing MxB. Here we showed that the reason why MxB does not block HIV-1-G208R is that MxB does not interact with HIV-1 cores bearing the mutation G208R. To understand whether MxB contributes to the HIV-1 restriction imposed by IFN-α- treated human cells, we challenged IFN-α-treated cells with HIV-G208R and found that MxB does not contribute to the restriction imposed by IFN-α-treated cells. To more directly test the contribution of MxB, we challenged IFN-α-treated human cells that are knocked out for the expression of MxB with HIV-1. These experiments suggested that MxB does not contribute to the HIV-1 restriction observed in IFN-α-treated human cells.

Original languageEnglish (US)
Pages (from-to)3056-3064
Number of pages9
JournalJournal of Virology
Volume90
Issue number6
DOIs
StatePublished - 2016

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Human immunodeficiency virus 1
HIV Infections
HIV-1
interferons
Interferons
infection
capsid
Capsid
cells
mutants
interferon-alpha
Interferon-alpha
HIV
mutation
Mutation

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

MxB is not responsible for the blocking of HIV-1 infection observed in alpha interferontreated cells. / Opp, Silvana; Vieira, Daniel A S A; Schulte, Bianca; Chanda, Sumit K.; Diaz-Griffero, Felipe.

In: Journal of Virology, Vol. 90, No. 6, 2016, p. 3056-3064.

Research output: Contribution to journalArticle

Opp, Silvana ; Vieira, Daniel A S A ; Schulte, Bianca ; Chanda, Sumit K. ; Diaz-Griffero, Felipe. / MxB is not responsible for the blocking of HIV-1 infection observed in alpha interferontreated cells. In: Journal of Virology. 2016 ; Vol. 90, No. 6. pp. 3056-3064.
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