MutS-mediated detection of DNA mismatches using atomic force microscopy

Hui (Herb) Sun, Hiroki Yokota

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We have developed an atomic force microscopy-based method for detecting DNA base-pair mismatches using MutS protein isolated from E. coli. MutS is a biological sensor and a locator of DNA base-pair mismatches. It binds specifically to a mismatched DNA base pair and initiates a process of DNA repair. To test the possibility of visually detecting mismatched base pairs by atomic force microscopy, we prepared DNA templates ~500 bp in length consisting of a single or multiple base-pair mismatches. We demonstrate that MutS binding sites on individual DNA molecules were readily detectable by atomic force microscopy and that the observed positions were in good agreement with the predicted sites of base-pairs mismatches at a few-nanometer resolution. The technique described here is rapid and sensitive and is expected to be useful in screening mutations and DNA polymorphisms.

Original languageEnglish (US)
Pages (from-to)3138-3141
Number of pages4
JournalAnalytical Chemistry
Volume72
Issue number14
DOIs
StatePublished - Jul 15 2000
Externally publishedYes

Fingerprint

Atomic force microscopy
DNA
Polymorphism
Escherichia coli
Screening
Repair
Binding Sites
Molecules
Sensors
Proteins

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

MutS-mediated detection of DNA mismatches using atomic force microscopy. / Sun, Hui (Herb); Yokota, Hiroki.

In: Analytical Chemistry, Vol. 72, No. 14, 15.07.2000, p. 3138-3141.

Research output: Contribution to journalArticle

@article{d80c6c8bbda04b788d191dee24433923,
title = "MutS-mediated detection of DNA mismatches using atomic force microscopy",
abstract = "We have developed an atomic force microscopy-based method for detecting DNA base-pair mismatches using MutS protein isolated from E. coli. MutS is a biological sensor and a locator of DNA base-pair mismatches. It binds specifically to a mismatched DNA base pair and initiates a process of DNA repair. To test the possibility of visually detecting mismatched base pairs by atomic force microscopy, we prepared DNA templates ~500 bp in length consisting of a single or multiple base-pair mismatches. We demonstrate that MutS binding sites on individual DNA molecules were readily detectable by atomic force microscopy and that the observed positions were in good agreement with the predicted sites of base-pairs mismatches at a few-nanometer resolution. The technique described here is rapid and sensitive and is expected to be useful in screening mutations and DNA polymorphisms.",
author = "Sun, {Hui (Herb)} and Hiroki Yokota",
year = "2000",
month = "7",
day = "15",
doi = "10.1021/ac991263i",
language = "English (US)",
volume = "72",
pages = "3138--3141",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "14",

}

TY - JOUR

T1 - MutS-mediated detection of DNA mismatches using atomic force microscopy

AU - Sun, Hui (Herb)

AU - Yokota, Hiroki

PY - 2000/7/15

Y1 - 2000/7/15

N2 - We have developed an atomic force microscopy-based method for detecting DNA base-pair mismatches using MutS protein isolated from E. coli. MutS is a biological sensor and a locator of DNA base-pair mismatches. It binds specifically to a mismatched DNA base pair and initiates a process of DNA repair. To test the possibility of visually detecting mismatched base pairs by atomic force microscopy, we prepared DNA templates ~500 bp in length consisting of a single or multiple base-pair mismatches. We demonstrate that MutS binding sites on individual DNA molecules were readily detectable by atomic force microscopy and that the observed positions were in good agreement with the predicted sites of base-pairs mismatches at a few-nanometer resolution. The technique described here is rapid and sensitive and is expected to be useful in screening mutations and DNA polymorphisms.

AB - We have developed an atomic force microscopy-based method for detecting DNA base-pair mismatches using MutS protein isolated from E. coli. MutS is a biological sensor and a locator of DNA base-pair mismatches. It binds specifically to a mismatched DNA base pair and initiates a process of DNA repair. To test the possibility of visually detecting mismatched base pairs by atomic force microscopy, we prepared DNA templates ~500 bp in length consisting of a single or multiple base-pair mismatches. We demonstrate that MutS binding sites on individual DNA molecules were readily detectable by atomic force microscopy and that the observed positions were in good agreement with the predicted sites of base-pairs mismatches at a few-nanometer resolution. The technique described here is rapid and sensitive and is expected to be useful in screening mutations and DNA polymorphisms.

UR - http://www.scopus.com/inward/record.url?scp=0001320568&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0001320568&partnerID=8YFLogxK

U2 - 10.1021/ac991263i

DO - 10.1021/ac991263i

M3 - Article

C2 - 10939378

AN - SCOPUS:0001320568

VL - 72

SP - 3138

EP - 3141

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 14

ER -