MutLγ promotes repeat expansion in a Fragile X mouse model while EXO1 is protective

Xiaonan Zhao, Yongwei Zhang, Kenneth Wilkins, Winfried Edelmann, Karen Usdin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The Fragile X-related disorders (FXDs) are Repeat Expansion Diseases resulting from an expansion of a CGG-repeat tract at the 5’ end of the FMR1 gene. The mechanism responsible for this unusual mutation is not fully understood. We have previously shown that mismatch repair (MMR) complexes, MSH2/MSH3 (MutSβ) and MSH2/MSH6 (MutSα), together with Polβ, a DNA polymerase important for base excision repair (BER), are important for expansions in a mouse model of these disorders. Here we show that MLH1/MLH3 (MutLγ), a protein complex that can act downstream of MutSβ in MMR, is also required for all germ line and somatic expansions. However, exonuclease I (EXO1), which acts downstream of MutL proteins in MMR, is not required. In fact, a null mutation in Exo1 results in more extensive germ line and somatic expansions than is seen in Exo1+/+ animals. Furthermore, mice homozygous for a point mutation (D173A) in Exo1 that eliminates its nuclease activity but retains its native conformation, shows a level of expansion that is intermediate between Exo1+/+ and Exo1-/- animals. Thus, our data suggests that expansion of the FX repeat in this mouse model occurs via a MutLγ-dependent, EXO1-independent pathway, with EXO1 protecting against expansion both in a nuclease-dependent and a nuclease-independent manner. Our data thus have implications for the expansion mechanism and add to our understanding of the genetic factors that may be modifiers of expansion risk in humans.

Original languageEnglish (US)
Article numbere1007719
JournalPLoS Genetics
Volume14
Issue number10
DOIs
StatePublished - Oct 1 2018

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DNA Mismatch Repair
nucleases
repair
animal models
Germ Cells
mutation
germ cells
Mutation
modifiers (genes)
DNA-directed DNA polymerase
DNA-Directed DNA Polymerase
point mutation
DNA repair
Point Mutation
DNA Repair
protein
animal
animals
proteins
mice

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

MutLγ promotes repeat expansion in a Fragile X mouse model while EXO1 is protective. / Zhao, Xiaonan; Zhang, Yongwei; Wilkins, Kenneth; Edelmann, Winfried; Usdin, Karen.

In: PLoS Genetics, Vol. 14, No. 10, e1007719, 01.10.2018.

Research output: Contribution to journalArticle

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