Mutations in the Caenorhabditis elegans β-tubulin gene mec-7

Effects on microtubule assembly and stability and on tubulin autoregulation

Cathy Savage, Yingzi Xue, Shohei Mitani, David H. Hall, Randa Zakhary, Martin Chalfie

Research output: Contribution to journalArticle

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Abstract

We have sequenced 45 mutations in mec-7, a β-tubulin gene required for the production of 15-protofilament microtubules in the nematode Caenorhabditis elegans, and have correlated sequence alterations with mutant phenotypes. The expression patterns of most alleles have also been determined by in situ hybridization and immunocytochemistry. Most (12/16) complete loss-of-function alleles, which are recessive, result from nonsense mutations, insertions, or deletions; three others disrupt a putative GTP-binding domain. Three of the four loss-of-function, missense mutations result in elevated mec-7 message levels, suggesting a defect in tubulin autoregulation that may be attributable to a loss in the ability to form heterodimers. Most (8/9) mild alleles are caused by missense mutations. Two mild alleles appear to increase microtubule stability and lead to the elaboration of ectopic neuronal processes in mec-7-expressing cells. Most (15/23) mutations that cause severe dominant or semidominant phenotypes are clustered into three discrete domains; four others occur in putative GTP-binding regions. Many of these dominant mutations appear to completely disrupt microtubule assembly.

Original languageEnglish (US)
Pages (from-to)2165-2175
Number of pages11
JournalJournal of Cell Science
Volume107
Issue number8
StatePublished - Aug 1994

Fingerprint

Caenorhabditis elegans
Tubulin
Microtubules
Homeostasis
Alleles
Mutation
Missense Mutation
Guanosine Triphosphate
Genes
Phenotype
Nonsense Codon
In Situ Hybridization
Immunohistochemistry

Keywords

  • β-tubulin autoregulation
  • β-tubulin mutation
  • Caenorhabditis elegans

ASJC Scopus subject areas

  • Cell Biology

Cite this

Mutations in the Caenorhabditis elegans β-tubulin gene mec-7 : Effects on microtubule assembly and stability and on tubulin autoregulation. / Savage, Cathy; Xue, Yingzi; Mitani, Shohei; Hall, David H.; Zakhary, Randa; Chalfie, Martin.

In: Journal of Cell Science, Vol. 107, No. 8, 08.1994, p. 2165-2175.

Research output: Contribution to journalArticle

Savage, Cathy ; Xue, Yingzi ; Mitani, Shohei ; Hall, David H. ; Zakhary, Randa ; Chalfie, Martin. / Mutations in the Caenorhabditis elegans β-tubulin gene mec-7 : Effects on microtubule assembly and stability and on tubulin autoregulation. In: Journal of Cell Science. 1994 ; Vol. 107, No. 8. pp. 2165-2175.
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