Mutations in a member of the ADAMTS gene family cause thrombotic thrombocytopenic purpura

Gallia G. Levy, William C. Nichols, Eric C. Lian, Tatiana Foroud, Jeanette N. McClintick, Beth M. McGee, Angela Y. Yang, David R. Siemieniak, Kenneth R. Stark, Ralph Gruppo, Ravindra Sarode, Susan B. Shurin, Visalam Chandrasekaran, Sally P. Stabler, Hernan Sabio, Eric E. Bouhassira, Jefferson D. Upshaw, David Ginsburg, Han Mou Tsai

Research output: Contribution to journalArticle

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Abstract

Thrombotic thrombocytopenic purpura (TTP) is a life-threatening systemic illness of abrupt onset and unknown cause. Proteolysis of the blood-clotting protein von Willebrand factor (VWF) observed in normal plasma is decreased in TTP patients. However, the identity of the responsible protease and its role in the pathophysiology of TTP remain unknown. We performed genome-wide linkage analysis in four pedigrees of humans with congenital TTP and mapped the responsible genetic locus to chromosome 9q34. A predicted gene in the identifed interval corresponds to a segment of a much larger transcript, identifying a new member of the ADAMTS family of zinc metalloproteinase genes (ADAMTS13). Analysis of patients' genomic DNA identified 12 mutations in the ADAMTS13 gene, accounting for 14 of the 15 disease alleles studied. We show that deficiency of ADAMTS13 is the molecular mechanism responsible for TTP, and suggest that physiologic proteolysis of VWF and/or other ADAMTS13 substrates is required for normal vascular homeostasis.

Original languageEnglish (US)
Pages (from-to)488-494
Number of pages7
JournalNature
Volume413
Issue number6855
DOIs
StatePublished - Oct 4 2001

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Thrombotic Thrombocytopenic Purpura
Mutation
Genes
von Willebrand Factor
Proteolysis
Genetic Loci
Metalloproteases
Blood Coagulation
Pedigree
Blood Vessels
Zinc
Blood Proteins
Homeostasis
Peptide Hydrolases
Chromosomes
Alleles
Genome
DNA

ASJC Scopus subject areas

  • General

Cite this

Levy, G. G., Nichols, W. C., Lian, E. C., Foroud, T., McClintick, J. N., McGee, B. M., ... Tsai, H. M. (2001). Mutations in a member of the ADAMTS gene family cause thrombotic thrombocytopenic purpura. Nature, 413(6855), 488-494. https://doi.org/10.1038/35097008

Mutations in a member of the ADAMTS gene family cause thrombotic thrombocytopenic purpura. / Levy, Gallia G.; Nichols, William C.; Lian, Eric C.; Foroud, Tatiana; McClintick, Jeanette N.; McGee, Beth M.; Yang, Angela Y.; Siemieniak, David R.; Stark, Kenneth R.; Gruppo, Ralph; Sarode, Ravindra; Shurin, Susan B.; Chandrasekaran, Visalam; Stabler, Sally P.; Sabio, Hernan; Bouhassira, Eric E.; Upshaw, Jefferson D.; Ginsburg, David; Tsai, Han Mou.

In: Nature, Vol. 413, No. 6855, 04.10.2001, p. 488-494.

Research output: Contribution to journalArticle

Levy, GG, Nichols, WC, Lian, EC, Foroud, T, McClintick, JN, McGee, BM, Yang, AY, Siemieniak, DR, Stark, KR, Gruppo, R, Sarode, R, Shurin, SB, Chandrasekaran, V, Stabler, SP, Sabio, H, Bouhassira, EE, Upshaw, JD, Ginsburg, D & Tsai, HM 2001, 'Mutations in a member of the ADAMTS gene family cause thrombotic thrombocytopenic purpura', Nature, vol. 413, no. 6855, pp. 488-494. https://doi.org/10.1038/35097008
Levy GG, Nichols WC, Lian EC, Foroud T, McClintick JN, McGee BM et al. Mutations in a member of the ADAMTS gene family cause thrombotic thrombocytopenic purpura. Nature. 2001 Oct 4;413(6855):488-494. https://doi.org/10.1038/35097008
Levy, Gallia G. ; Nichols, William C. ; Lian, Eric C. ; Foroud, Tatiana ; McClintick, Jeanette N. ; McGee, Beth M. ; Yang, Angela Y. ; Siemieniak, David R. ; Stark, Kenneth R. ; Gruppo, Ralph ; Sarode, Ravindra ; Shurin, Susan B. ; Chandrasekaran, Visalam ; Stabler, Sally P. ; Sabio, Hernan ; Bouhassira, Eric E. ; Upshaw, Jefferson D. ; Ginsburg, David ; Tsai, Han Mou. / Mutations in a member of the ADAMTS gene family cause thrombotic thrombocytopenic purpura. In: Nature. 2001 ; Vol. 413, No. 6855. pp. 488-494.
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