Mutations affecting excision of the intron from a eukaryotic dimeric tRNA precursor.

Ian M. Willis, H. Hottinger, D. Pearson, V. Chisholm, U. Leupold, D. Söll

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The nucleotide sequences of a Schizosaccharomyces pombe opal suppressor serine tRNA gene (sup9-e) and of 12 in vivo-generated mutant genes, which have lost the ability to suppress UGA mutations, have been determined. Analysis of the expression of these genes in Saccharomyces cerevisiae in vitro and in vivo systems has revealed defects in tRNA gene transcription and precursor tRNA processing. Single base changes in the D-loop, the intron and the extra arm affect the efficiency of splicing of the tRNA precursors while an anti-codon stem mutation may affect the accuracy of this process. Two mutations which occur in the intervening sequence of the sup9-e gene allow an alternate tRNA base pairing configuration. Transcription of the sup9-e gene and of the adjacent tRNAMet gene (located 7 bp downstream) is essentially abolished in vivo by a G----A19 mutation in the tRNASer gene, suggesting that tRNAMet may be derived solely via processing of the tRNASer-tRNAMet dimeric precursor.

Original languageEnglish (US)
Pages (from-to)1573-1580
Number of pages8
JournalEMBO Journal
Volume3
Issue number7
StatePublished - Jul 1984
Externally publishedYes

Fingerprint

RNA Precursors
Introns
Genes
Mutation
RNA, Transfer, Met
RNA, Transfer, Ser
Transfer RNA
Transcription
Schizosaccharomyces
Codon
Base Pairing
Serine
Processing
Saccharomyces cerevisiae
Yeast
Nucleotides
Gene Expression
Defects

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Willis, I. M., Hottinger, H., Pearson, D., Chisholm, V., Leupold, U., & Söll, D. (1984). Mutations affecting excision of the intron from a eukaryotic dimeric tRNA precursor. EMBO Journal, 3(7), 1573-1580.

Mutations affecting excision of the intron from a eukaryotic dimeric tRNA precursor. / Willis, Ian M.; Hottinger, H.; Pearson, D.; Chisholm, V.; Leupold, U.; Söll, D.

In: EMBO Journal, Vol. 3, No. 7, 07.1984, p. 1573-1580.

Research output: Contribution to journalArticle

Willis, IM, Hottinger, H, Pearson, D, Chisholm, V, Leupold, U & Söll, D 1984, 'Mutations affecting excision of the intron from a eukaryotic dimeric tRNA precursor.', EMBO Journal, vol. 3, no. 7, pp. 1573-1580.
Willis IM, Hottinger H, Pearson D, Chisholm V, Leupold U, Söll D. Mutations affecting excision of the intron from a eukaryotic dimeric tRNA precursor. EMBO Journal. 1984 Jul;3(7):1573-1580.
Willis, Ian M. ; Hottinger, H. ; Pearson, D. ; Chisholm, V. ; Leupold, U. ; Söll, D. / Mutations affecting excision of the intron from a eukaryotic dimeric tRNA precursor. In: EMBO Journal. 1984 ; Vol. 3, No. 7. pp. 1573-1580.
@article{dac98c54b8c643e69cb15ec5c2269fe2,
title = "Mutations affecting excision of the intron from a eukaryotic dimeric tRNA precursor.",
abstract = "The nucleotide sequences of a Schizosaccharomyces pombe opal suppressor serine tRNA gene (sup9-e) and of 12 in vivo-generated mutant genes, which have lost the ability to suppress UGA mutations, have been determined. Analysis of the expression of these genes in Saccharomyces cerevisiae in vitro and in vivo systems has revealed defects in tRNA gene transcription and precursor tRNA processing. Single base changes in the D-loop, the intron and the extra arm affect the efficiency of splicing of the tRNA precursors while an anti-codon stem mutation may affect the accuracy of this process. Two mutations which occur in the intervening sequence of the sup9-e gene allow an alternate tRNA base pairing configuration. Transcription of the sup9-e gene and of the adjacent tRNAMet gene (located 7 bp downstream) is essentially abolished in vivo by a G----A19 mutation in the tRNASer gene, suggesting that tRNAMet may be derived solely via processing of the tRNASer-tRNAMet dimeric precursor.",
author = "Willis, {Ian M.} and H. Hottinger and D. Pearson and V. Chisholm and U. Leupold and D. S{\"o}ll",
year = "1984",
month = "7",
language = "English (US)",
volume = "3",
pages = "1573--1580",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "7",

}

TY - JOUR

T1 - Mutations affecting excision of the intron from a eukaryotic dimeric tRNA precursor.

AU - Willis, Ian M.

AU - Hottinger, H.

AU - Pearson, D.

AU - Chisholm, V.

AU - Leupold, U.

AU - Söll, D.

PY - 1984/7

Y1 - 1984/7

N2 - The nucleotide sequences of a Schizosaccharomyces pombe opal suppressor serine tRNA gene (sup9-e) and of 12 in vivo-generated mutant genes, which have lost the ability to suppress UGA mutations, have been determined. Analysis of the expression of these genes in Saccharomyces cerevisiae in vitro and in vivo systems has revealed defects in tRNA gene transcription and precursor tRNA processing. Single base changes in the D-loop, the intron and the extra arm affect the efficiency of splicing of the tRNA precursors while an anti-codon stem mutation may affect the accuracy of this process. Two mutations which occur in the intervening sequence of the sup9-e gene allow an alternate tRNA base pairing configuration. Transcription of the sup9-e gene and of the adjacent tRNAMet gene (located 7 bp downstream) is essentially abolished in vivo by a G----A19 mutation in the tRNASer gene, suggesting that tRNAMet may be derived solely via processing of the tRNASer-tRNAMet dimeric precursor.

AB - The nucleotide sequences of a Schizosaccharomyces pombe opal suppressor serine tRNA gene (sup9-e) and of 12 in vivo-generated mutant genes, which have lost the ability to suppress UGA mutations, have been determined. Analysis of the expression of these genes in Saccharomyces cerevisiae in vitro and in vivo systems has revealed defects in tRNA gene transcription and precursor tRNA processing. Single base changes in the D-loop, the intron and the extra arm affect the efficiency of splicing of the tRNA precursors while an anti-codon stem mutation may affect the accuracy of this process. Two mutations which occur in the intervening sequence of the sup9-e gene allow an alternate tRNA base pairing configuration. Transcription of the sup9-e gene and of the adjacent tRNAMet gene (located 7 bp downstream) is essentially abolished in vivo by a G----A19 mutation in the tRNASer gene, suggesting that tRNAMet may be derived solely via processing of the tRNASer-tRNAMet dimeric precursor.

UR - http://www.scopus.com/inward/record.url?scp=0021456848&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021456848&partnerID=8YFLogxK

M3 - Article

C2 - 6430697

AN - SCOPUS:0021456848

VL - 3

SP - 1573

EP - 1580

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 7

ER -