Mutational and functional analysis of Large in a novel CHO glycosylation mutant

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Inactivating mutations of Large reduce the functional glycosylation of α-dystroglycan (α-DG) and lead to muscular dystrophy in mouse and humans. The N-terminal domain of Large is most similar to UDP-glucose glucosyltransferases (UGGT), and the C-terminal domain is related to the human i blood group transferase β1,3GlcNAcT-1. The amino acids at conserved motifs DQD+1 and DQD+3 in the UGGT domain are necessary for mammalian UGGT activity. When the corresponding residues were mutated to Ala in mouse Large, α-DG was not functionally glycosylated. A similar result was obtained when a DXD motif in the β1,3GlcNAcT-1 domain was mutated to AIA. Therefore, the first putative glycosyltransferase domain of Large has properties of a UGGT and the second of a typical glycosyltransferase. Co-transfection of Large mutants affected in the different glycosyltransferase domains did not lead to complementation. While Large mutants were more localized to the endoplasmic reticulum than wild-type Large or revertants, all mutants were in the Golgi, and only very low levels of Golgi-localized Large were necessary to generate functional α-DG. When Large was overexpressed in ldlD.Lec1 mutant Chinese hamster ovary (CHO) cells which synthesize few, if any, mucin O-GalNAc glycans and no complex N-glycans, functional α-DG was produced, presumably by modifying O-mannose glycans. To investigate mucin O-GalNAc glycans as substrates of Large, a new CHO mutant Lec15.Lec1 that lacked O-mannose and complex N-glycans was isolated and characterized. Following transfection with Large, Lec15.Lec1 cells also generated functionally glycosylated α-DG. Thus, Large may act on the O-mannose, complex N-glycans and mucin O-GalNAc glycans of α-DG.

Original languageEnglish (US)
Pages (from-to)971-986
Number of pages16
JournalGlycobiology
Volume19
Issue number9
DOIs
StatePublished - Aug 20 2009

    Fingerprint

Keywords

  • CHO mutants
  • DXD
  • Laminin
  • Large
  • Mutagenesis
  • α-dystroglycan

ASJC Scopus subject areas

  • Biochemistry

Cite this