Mutational analysis of subunit iβ2 (MECL-1) demonstrates conservation of cleavage specificity between yeast and mammalian proteasomes

Ulrike Salzmann, Sylvie Kral, Beate Braun, Sybille Standera, Marion Schmidt, Peter M. Kloetzel, Alice Sijts

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Proteasomes are the major protein-degrading complexes in the cytosol and regulate many cellular processes. To examine the functional importance of the MC14/MECL-1 proteasome active site subunits, cell lines expressing a catalytically inactive form of MECL-1 were established. Whereas mutant MECL-1 was readily incorporated into cytosolic proteasomes, replacing the constitutive MC14 subunit, removal of the prosequence was incomplete indicating that its processing required autocatalytic cleavage. Functional analyses showed that the absence of the MC14/MECL-1 active sites abrogated proteasomal trypsin-like activity, but did not affect other catalytic activities. Our data demonstrate a conservation of cleavage specificity between mammalian and yeast proteasomes. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)11-15
Number of pages5
JournalFEBS Letters
Volume454
Issue number1-2
DOIs
StatePublished - Jul 2 1999
Externally publishedYes

Fingerprint

Proteasome Endopeptidase Complex
Yeast
Conservation
Yeasts
Catalytic Domain
Cytosol
Trypsin
Catalyst activity
Cells
Cell Line
Processing
Proteins

Keywords

  • 20S proteasome
  • Autocatalytic processing
  • Interferon γ-inducible subunit
  • MECL-1 T1A
  • Peptide hydrolysis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Mutational analysis of subunit iβ2 (MECL-1) demonstrates conservation of cleavage specificity between yeast and mammalian proteasomes. / Salzmann, Ulrike; Kral, Sylvie; Braun, Beate; Standera, Sybille; Schmidt, Marion; Kloetzel, Peter M.; Sijts, Alice.

In: FEBS Letters, Vol. 454, No. 1-2, 02.07.1999, p. 11-15.

Research output: Contribution to journalArticle

Salzmann, Ulrike ; Kral, Sylvie ; Braun, Beate ; Standera, Sybille ; Schmidt, Marion ; Kloetzel, Peter M. ; Sijts, Alice. / Mutational analysis of subunit iβ2 (MECL-1) demonstrates conservation of cleavage specificity between yeast and mammalian proteasomes. In: FEBS Letters. 1999 ; Vol. 454, No. 1-2. pp. 11-15.
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AU - Schmidt, Marion

AU - Kloetzel, Peter M.

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