Mutation of mouse Mayp/Pstpip2 causes a macrophage autoinflammatory disease

Johannes Grosse, Violeta Chitu, Andreas Marquardt, Petra Hanke, Carolin Schmittwolf, Lutz Zeitlmann, Patricia Schropp, Bettina Barth, Philipp Yu, Rainer Paffenholz, Gabriele Stumm, Michael Nehls, E. Richard Stanley

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101 Scopus citations

Abstract

Macrophage actin-associated tyrosine phosphorylated protein (MAYP)/PSTPIP2, a PCH protein, is involved in the regulation of macrophage motility. Mutations in a closely related gene, PSTPIP1/CD2BP1, cause a dominantly inherited autoinflammatory disorder known as PAPA syndrome. A mutant mouse obtained by chemical mutagenesis exhibited an autoinflammatory disorder characterized by macrophage infiltration and inflammation, leading to osteolysis and necrosis in paws and necrosis of ears. Positional cloning of this recessive mutation, termed Lupo, identified a T to A nucleotide exchange leading to an amino acid substitution (I282N) in the sequence of MAYP. MaypLp/Lp disease was transferable by bone marrow transplantation and developed in the absence of lymphocytes. Consistent with the involvement of macrophages, lesion development could be prevented by the administration of clodronate liposomes. MAYP is expressed in monocytes/macrophages and in a Mac1+ subfraction of granulocytes. LPS stimulation increases its expression in macrophages. Because of the instability of the mutant protein, MAYP expression is reduced 3-fold in MaypLp/Lp macrophages and, on LPS stimulation, does not rise above the level of unstimulated wild-type (WT) cells. MaypLp/Lp mice expressed elevated circulating levels of several cytokines, including MCP-1; their macrophages exhibited altered cytokine production in vitro. These studies suggest that MAYP plays an anti-inflammatory role in macrophages.

Original languageEnglish (US)
Pages (from-to)3350-3358
Number of pages9
JournalBlood
Volume107
Issue number8
DOIs
Publication statusPublished - Apr 15 2006

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ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Grosse, J., Chitu, V., Marquardt, A., Hanke, P., Schmittwolf, C., Zeitlmann, L., ... Stanley, E. R. (2006). Mutation of mouse Mayp/Pstpip2 causes a macrophage autoinflammatory disease. Blood, 107(8), 3350-3358. https://doi.org/10.1182/blood-2005-09-3556