Mutation frequency and type during ageing in mouse seminiferous tubules

Sandra L. Martin, Craig L. Hopkins, Anne Naumer, Martijn E.T. Dollé, Jan Vijg

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Mutations arise in the germline by errors of replication, recombination and repair, and the movement of transposable elements. Transgenic mice bearing reporter genes such as lacZ have proven useful for measurements of spontaneous and induced mutation frequencies, as well as studies of the effects of ageing. In this study, testicular DNA from lacZ transgenic mice was examined for age-related effects on mutation frequency and type. The recovered transgene was tested for simple substitutions and rearrangements including transposition of endogenous mobile elements. There was no evidence for either an age-related accumulation of mutations, or for the insertion of retrotransposons into the lacZ reporter gene in the testis. We conclude that the frequency of retrotransposition of several mouse mobile elements into the lacZ reporter gene is less than 3.73 × 10-8. This is significantly less than the known frequency of ∼ 7% of all spontaneous mutations in the mouse being due to retrotransposition of these elements.

Original languageEnglish (US)
Pages (from-to)1321-1331
Number of pages11
JournalMechanisms of Ageing and Development
Volume122
Issue number12
DOIs
StatePublished - Jul 7 2001
Externally publishedYes

Keywords

  • IAP
  • LINE-1
  • SINE

ASJC Scopus subject areas

  • Aging
  • Developmental Biology

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