Murine and human 3' IgH regulatory sequences

B. K. Birshtein, C. Chen, S. Saleque, J. S. Michaelson, M. Singh, R. D. Little

Research output: Contribution to journalArticle

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Abstract

Both murine plasmacytomas and human Burkitt lymphomas contain chromosomal translocations involving the c-myc gene and the Ig gene cluster. As a result of the translocation in mouse plasmacytomas, the myc oncogene comes under the influence of regulatory sequences 3 of the murine IgH gene cluster. The murine 3 regulatory region contains four enhancers, three of which are located in a 25 kb unit of dyad symmetry [8, 14]. This unit contains a central enhancer, flanked by two identical enhancers at the termini, and we have speculated that this unit has entered the mouse genome during evolution as a transposable element. The fourth, and most downstream, enhancer comprises a separate structural and functional unit. Synergy among these enhancers has been documented and it has been suggested that these elements comprise a locus control region [6]. We have identified two virtually identical human enhancers 3 of the IgH locus, one downstream of the Cα1 gene and one downstream of the Cα2 gene. These bear a region of homology to one of the mouse 3 enhancers, and are predicted to play a role in myc ontogenesis. Preliminary evidence for DNA rearrangements of 3 regulatory sequences in human myeloma cell lines is presented.

Original languageEnglish (US)
Pages (from-to)73-80
Number of pages8
JournalCurrent Topics in Microbiology and Immunology
Volume224
StatePublished - 1997

Fingerprint

myc Genes
Plasmacytoma
Multigene Family
Locus Control Region
Genetic Translocation
Immunoglobulin Genes
DNA Transposable Elements
Burkitt Lymphoma
Gene Rearrangement
Nucleic Acid Regulatory Sequences
Genes
Genome
Cell Line

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology (medical)
  • Immunology and Microbiology(all)
  • Microbiology

Cite this

Birshtein, B. K., Chen, C., Saleque, S., Michaelson, J. S., Singh, M., & Little, R. D. (1997). Murine and human 3' IgH regulatory sequences. Current Topics in Microbiology and Immunology, 224, 73-80.

Murine and human 3' IgH regulatory sequences. / Birshtein, B. K.; Chen, C.; Saleque, S.; Michaelson, J. S.; Singh, M.; Little, R. D.

In: Current Topics in Microbiology and Immunology, Vol. 224, 1997, p. 73-80.

Research output: Contribution to journalArticle

Birshtein, BK, Chen, C, Saleque, S, Michaelson, JS, Singh, M & Little, RD 1997, 'Murine and human 3' IgH regulatory sequences', Current Topics in Microbiology and Immunology, vol. 224, pp. 73-80.
Birshtein BK, Chen C, Saleque S, Michaelson JS, Singh M, Little RD. Murine and human 3' IgH regulatory sequences. Current Topics in Microbiology and Immunology. 1997;224:73-80.
Birshtein, B. K. ; Chen, C. ; Saleque, S. ; Michaelson, J. S. ; Singh, M. ; Little, R. D. / Murine and human 3' IgH regulatory sequences. In: Current Topics in Microbiology and Immunology. 1997 ; Vol. 224. pp. 73-80.
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