Multivalent Lectin—Carbohydrate Interactions: Energetics and Mechanisms of Binding

Tarun K. Dam, C. Fred Brewer

Research output: Chapter in Book/Report/Conference proceedingChapter

91 Scopus citations

Abstract

The biological signaling properties of lectins, which are carbohydrate-binding proteins, are due to their ability to bind and cross-link multivalent glycoprotein receptors on the surface of normal and transformed cells. While the crosslinking properties of lectins with multivalent carbohydrates and glycoproteins are relatively well understood, the mechanisms of binding of lectins to multivalent glycoconjugates are less well understood. Recently, the thermodynamics of binding of lectins to synthetic clustered glycosides, a multivalent globular glycoprotein, and to linear glycoproteins (mucins) have been described. The results are consistent with a dynamic binding mechanism in which lectins bind and jump from carbohydrate to carbohydrate epitope in these molecules. Importantly, the mechanism of binding of lectins to mucins is similar to that for a variety of protein ligands binding to DNA. Recent analysis also shows that high-affinity lectin–mucin crosslinking interactions are driven by favorable entropy of binding that is associated with the bind and jump mechanism. The results suggest that the binding of ligands to biopolymers, in general, may involve a common mechanism that involves enhanced entropic effects which facilitate binding and subsequent complex formation including enzymology.

Original languageEnglish (US)
Title of host publicationAdvances in Carbohydrate Chemistry and Biochemistry
PublisherAcademic Press Inc.
Pages139-164
Number of pages26
DOIs
StatePublished - Jan 2010

Publication series

NameAdvances in Carbohydrate Chemistry and Biochemistry
Volume63
ISSN (Print)0065-2318

Keywords

  • Glycoproteins
  • Lectins
  • Mucins
  • Multivalent carbohydrates
  • Thermodynamics

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

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