Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone

Liang Chen, Kalyan D. Chavda, José R. Mediavilla, Yanan Zhao, Henry S. Fraimow, Stephen G. Jenkins, Michael H. Levi, Tao Hong, Albert D. Rojtman, Christine C. Ginocchio, Robert A. Bonomo, Barry N. Kreiswirth

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

We describe a multiplex real-time PCR assay capable of identifying both the epidemic Klebsiella pneumoniae ST258 clone and blaKPC carbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100%) and specificity (100%) for identification of ST258 clone and blaKPC in a collection of 75 K. pneumoniae isolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistant K. pneumoniae clinical isolates.

Original languageEnglish (US)
Pages (from-to)3444-3447
Number of pages4
JournalAntimicrobial agents and chemotherapy
Volume56
Issue number6
DOIs
StatePublished - Jun 2012

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone'. Together they form a unique fingerprint.

Cite this