We describe a multiplex real-time PCR assay capable of identifying both the epidemic Klebsiella pneumoniae ST258 clone and blaKPC carbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100%) and specificity (100%) for identification of ST258 clone and blaKPC in a collection of 75 K. pneumoniae isolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistant K. pneumoniae clinical isolates.
|Original language||English (US)|
|Number of pages||4|
|Journal||Antimicrobial agents and chemotherapy|
|State||Published - Jun 2012|
ASJC Scopus subject areas
- Pharmacology (medical)
- Infectious Diseases