Multiple Kinesin Motors Coordinate Cytoplasmic RNA Transport on a Subpopulation of Microtubules in Xenopus Oocytes

Timothy J. Messitt, James A. Gagnon, Jill A. Kreiling, Catherine A. Pratt, Young J. Yoon, Kimberly L. Mowry

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

RNA localization is a widely conserved mechanism for generating cellular asymmetry. In Xenopus oocytes, microtubule-dependent transport of RNAs to the vegetal cortex underlies germ layer patterning. Although kinesin motors have been implicated in this process, the apparent polarity of the microtubule cytoskeleton has pointed instead to roles for minus-end-directed motors. To resolve this issue, we have analyzed participation of kinesin motors in vegetal RNA transport and identified a direct role for Xenopus kinesin-1. Moreover, in vivo interference and biochemical experiments reveal a key function for multiple motors, specifically kinesin-1 and kinesin-2, and suggest that these motors may interact during transport. Critically, we have discovered a subpopulation of microtubules with plus ends at the vegetal cortex, supporting roles for these kinesin motors in vegetal RNA transport. These results provide a new mechanistic basis for understanding directed RNA transport within the cytoplasm.

Original languageEnglish (US)
Pages (from-to)426-436
Number of pages11
JournalDevelopmental Cell
Volume15
Issue number3
DOIs
StatePublished - Sep 16 2008
Externally publishedYes

Fingerprint

RNA Transport
Kinesin
Xenopus
Microtubules
Oocytes
RNA
Germ Layers
Cytoskeleton
Cytoplasm

Keywords

  • CELLBIO
  • DEVBIO
  • RNA

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Multiple Kinesin Motors Coordinate Cytoplasmic RNA Transport on a Subpopulation of Microtubules in Xenopus Oocytes. / Messitt, Timothy J.; Gagnon, James A.; Kreiling, Jill A.; Pratt, Catherine A.; Yoon, Young J.; Mowry, Kimberly L.

In: Developmental Cell, Vol. 15, No. 3, 16.09.2008, p. 426-436.

Research output: Contribution to journalArticle

Messitt, Timothy J. ; Gagnon, James A. ; Kreiling, Jill A. ; Pratt, Catherine A. ; Yoon, Young J. ; Mowry, Kimberly L. / Multiple Kinesin Motors Coordinate Cytoplasmic RNA Transport on a Subpopulation of Microtubules in Xenopus Oocytes. In: Developmental Cell. 2008 ; Vol. 15, No. 3. pp. 426-436.
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