Multiple fragment ligation on glass surface

a novel approach.

Radhashree Maitra, A. R. Thakur

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Binding of DNA to pure silicon dioxide involving formation of hydrogen bonds by disruption of the hydration shell of DNA using chaotropic agents can be easily reversed with water or buffers of low ionic strength. When powdered glass was used instead of pure silicon dioxide the binding of DNA to glass was less easily reversed. DNA bound to glass permits digestion by restriction enzyme, ligation and transformation. The method opens up the possibility of enhancing transformation efficiency especially for DNA digests containing more than two pieces.

Original languageEnglish (US)
Pages (from-to)97-99
Number of pages3
JournalIndian Journal of Biochemistry and Biophysics
Volume31
Issue number2
StatePublished - Apr 1994
Externally publishedYes

Fingerprint

Glass
Ligation
DNA
Silicon Dioxide
Ionic strength
Hydration
Osmolar Concentration
Digestion
Hydrogen
Hydrogen bonds
Buffers
Water
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Multiple fragment ligation on glass surface : a novel approach. / Maitra, Radhashree; Thakur, A. R.

In: Indian Journal of Biochemistry and Biophysics, Vol. 31, No. 2, 04.1994, p. 97-99.

Research output: Contribution to journalArticle

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