TY - JOUR
T1 - Multiphoton intravital microscopy of rodents
AU - Scheele, Colinda L.G.J.
AU - Herrmann, David
AU - Yamashita, Erika
AU - Lo Celso, Cristina
AU - Jenne, Craig N.
AU - Oktay, Maja H.
AU - Entenberg, David
AU - Friedl, Peter
AU - Weigert, Roberto
AU - Meijboom, Franck L.B.
AU - Ishii, Masaru
AU - Timpson, Paul
AU - van Rheenen, Jacco
N1 - Publisher Copyright:
© 2022, Springer Nature Limited.
PY - 2022/12
Y1 - 2022/12
N2 - Tissues are heterogeneous with respect to cellular and non-cellular components and in the dynamic interactions between these elements. To study the behaviour and fate of individual cells in these complex tissues, intravital microscopy (IVM) techniques such as multiphoton microscopy have been developed to visualize intact and live tissues at cellular and subcellular resolution. IVM experiments have revealed unique insights into the dynamic interplay between different cell types and their local environment, and how this drives morphogenesis and homeostasis of tissues, inflammation and immune responses, and the development of various diseases. This Primer introduces researchers to IVM technologies, with a focus on multiphoton microscopy of rodents, and discusses challenges, solutions and practical tips on how to perform IVM. To illustrate the unique potential of IVM, several examples of results are highlighted. Finally, we discuss data reproducibility and how to handle big imaging data sets.
AB - Tissues are heterogeneous with respect to cellular and non-cellular components and in the dynamic interactions between these elements. To study the behaviour and fate of individual cells in these complex tissues, intravital microscopy (IVM) techniques such as multiphoton microscopy have been developed to visualize intact and live tissues at cellular and subcellular resolution. IVM experiments have revealed unique insights into the dynamic interplay between different cell types and their local environment, and how this drives morphogenesis and homeostasis of tissues, inflammation and immune responses, and the development of various diseases. This Primer introduces researchers to IVM technologies, with a focus on multiphoton microscopy of rodents, and discusses challenges, solutions and practical tips on how to perform IVM. To illustrate the unique potential of IVM, several examples of results are highlighted. Finally, we discuss data reproducibility and how to handle big imaging data sets.
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U2 - 10.1038/s43586-022-00168-w
DO - 10.1038/s43586-022-00168-w
M3 - Article
AN - SCOPUS:85141847297
SN - 2662-8449
VL - 2
JO - Nature Reviews Methods Primers
JF - Nature Reviews Methods Primers
IS - 1
M1 - 89
ER -