Multiphoton intravital microscopy of rodents

Colinda L.G.J. Scheele, David Herrmann, Erika Yamashita, Cristina Lo Celso, Craig N. Jenne, Maja H. Oktay, David Entenberg, Peter Friedl, Roberto Weigert, Franck L.B. Meijboom, Masaru Ishii, Paul Timpson, Jacco van Rheenen

Research output: Contribution to journalArticlepeer-review


Tissues are heterogeneous with respect to cellular and non-cellular components and in the dynamic interactions between these elements. To study the behaviour and fate of individual cells in these complex tissues, intravital microscopy (IVM) techniques such as multiphoton microscopy have been developed to visualize intact and live tissues at cellular and subcellular resolution. IVM experiments have revealed unique insights into the dynamic interplay between different cell types and their local environment, and how this drives morphogenesis and homeostasis of tissues, inflammation and immune responses, and the development of various diseases. This Primer introduces researchers to IVM technologies, with a focus on multiphoton microscopy of rodents, and discusses challenges, solutions and practical tips on how to perform IVM. To illustrate the unique potential of IVM, several examples of results are highlighted. Finally, we discuss data reproducibility and how to handle big imaging data sets.

Original languageEnglish (US)
Article number89
JournalNature Reviews Methods Primers
Issue number1
StatePublished - Dec 2022

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)


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