Multimodal microscopy of immune cells and melanoma for longitudinal studies

David Entenberg, Iana Aranda, Yongbiao Li, Ricardo Toledo-Crow, David Schaer, Yanyun Li

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Scopus citations

Abstract

Interavital microscopy of cancer is a well established tool that provides direct visualization of the tumor cycle. It traditionally involves one of several strategies: invasive subcutaneous (SC) implantation of tumors followed by surgical opening of skin flaps for imaging, techniques utilizing skin fold chambers and implanted optical windows or intradermal injections under 200μm from the skin surface. All of these techniques allow the use of fluorescent proteins as markers for biologically significant constituents. However, observation methods utilizing skin-flaps, skin-fold chambers and optical windows are invasive and tend to alter the immune environment of the tissue and/or limit the duration of studies that can be performed. If implanted correctly, intradermally injected rumors can be minimally invasive, will not require biopsies or surgical intervention to observe and are accessible for direct transdermal imaging with a number of in vivo modalities. We present our work in the development of a small animal intravital microscopy workstation that allows the acquisition of different contrast imaging modalities: reflectance confocal, wide field epifluorescence, multiphoton and second harmonic generation (SHG). The images are acquired pair-wise simultaneously and sequentially in time. The aim of our instrumentation is to gather all information generated by the single probing beam via the reflected or back-scattered signal, SHG signal and various fluorescence signals. Additionally, we also present our development of a microscopic tissue navigation technique to mark, label and track sites of interest. This technique enables us to revisit sites periodically and record, with different imaging contrasts, their biological changes over time.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
DOIs
StatePublished - May 11 2006
Externally publishedYes
EventMultimodal Biomedical Imaging - San Jose, CA, United States
Duration: Jan 21 2006Jan 24 2006

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume6081
ISSN (Print)0277-786X

Other

OtherMultimodal Biomedical Imaging
CountryUnited States
CitySan Jose, CA
Period1/21/061/24/06

Keywords

  • 2 photon
  • GFP
  • Longitudinal Melanoma Study
  • Multimodal Intravital Microscopy
  • Navigation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Entenberg, D., Aranda, I., Li, Y., Toledo-Crow, R., Schaer, D., & Li, Y. (2006). Multimodal microscopy of immune cells and melanoma for longitudinal studies. In Proceedings of SPIE - The International Society for Optical Engineering [60810A] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 6081). https://doi.org/10.1117/12.646907