Multifluorescence live analysis of herpes simplex virus type-1 replication

Michael Seyffert, Anna Paula de Oliveira, Cornel Fraefel, Rebecca Vogel

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The possibility to label specific viral and cellular structures with live cell markers such as autofl uorescent proteins has greatly contributed to our understanding of diverse steps of the virus life cycle, as it allows monitoring virus replication in a spatial and temporal fashion. Here, we describe the multi-fluorescent analysis of the multi-compartment herpes simplex virus type-1 by live-cell confocal laser scanning microscopy.

Original languageEnglish (US)
Pages (from-to)235-247
Number of pages13
JournalMethods in Molecular Biology
Volume1144
DOIs
StatePublished - 2014
Externally publishedYes

Keywords

  • Confocal laser scanning microscopy
  • Image processing
  • Live-cell imaging
  • Multi-fluorescent recombinant HSV-1

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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