The possibility to label specific viral and cellular structures with live cell markers such as autofl uorescent proteins has greatly contributed to our understanding of diverse steps of the virus life cycle, as it allows monitoring virus replication in a spatial and temporal fashion. Here, we describe the multi-fluorescent analysis of the multi-compartment herpes simplex virus type-1 by live-cell confocal laser scanning microscopy.
- Confocal laser scanning microscopy
- Image processing
- Live-cell imaging
- Multi-fluorescent recombinant HSV-1
ASJC Scopus subject areas
- Molecular Biology