Multifluorescence live analysis of herpes simplex virus type-1 replication

Michael Seyffert; Anna Paula de Oliveira; Cornel Fraefel; Rebecca Vogel

doi:10.1007/978-1-4939-0428-0_16

Multifluorescence live analysis of herpes simplex virus type-1 replication

Michael Seyffert, Anna Paula de Oliveira, Cornel Fraefel, Rebecca Vogel

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The possibility to label specific viral and cellular structures with live cell markers such as autofl uorescent proteins has greatly contributed to our understanding of diverse steps of the virus life cycle, as it allows monitoring virus replication in a spatial and temporal fashion. Here, we describe the multi-fluorescent analysis of the multi-compartment herpes simplex virus type-1 by live-cell confocal laser scanning microscopy.

Original language	English (US)
Pages (from-to)	235-247
Number of pages	13
Journal	Methods in Molecular Biology
Volume	1144
DOIs	https://doi.org/10.1007/978-1-4939-0428-0_16
State	Published - 2014
Externally published	Yes

Keywords

Confocal laser scanning microscopy
Image processing
Live-cell imaging
Multi-fluorescent recombinant HSV-1

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-0428-0_16

Cite this

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title = "Multifluorescence live analysis of herpes simplex virus type-1 replication",

abstract = "The possibility to label specific viral and cellular structures with live cell markers such as autofl uorescent proteins has greatly contributed to our understanding of diverse steps of the virus life cycle, as it allows monitoring virus replication in a spatial and temporal fashion. Here, we describe the multi-fluorescent analysis of the multi-compartment herpes simplex virus type-1 by live-cell confocal laser scanning microscopy.",

keywords = "Confocal laser scanning microscopy, Image processing, Live-cell imaging, Multi-fluorescent recombinant HSV-1",

author = "Michael Seyffert and {de Oliveira}, {Anna Paula} and Cornel Fraefel and Rebecca Vogel",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2014.",

year = "2014",

doi = "10.1007/978-1-4939-0428-0_16",

language = "English (US)",

volume = "1144",

pages = "235--247",

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AU - Seyffert, Michael

AU - de Oliveira, Anna Paula

AU - Fraefel, Cornel

AU - Vogel, Rebecca

N1 - Publisher Copyright: © Springer Science+Business Media New York 2014.

PY - 2014

Y1 - 2014

N2 - The possibility to label specific viral and cellular structures with live cell markers such as autofl uorescent proteins has greatly contributed to our understanding of diverse steps of the virus life cycle, as it allows monitoring virus replication in a spatial and temporal fashion. Here, we describe the multi-fluorescent analysis of the multi-compartment herpes simplex virus type-1 by live-cell confocal laser scanning microscopy.

AB - The possibility to label specific viral and cellular structures with live cell markers such as autofl uorescent proteins has greatly contributed to our understanding of diverse steps of the virus life cycle, as it allows monitoring virus replication in a spatial and temporal fashion. Here, we describe the multi-fluorescent analysis of the multi-compartment herpes simplex virus type-1 by live-cell confocal laser scanning microscopy.

KW - Confocal laser scanning microscopy

KW - Image processing

KW - Live-cell imaging

KW - Multi-fluorescent recombinant HSV-1

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SP - 235

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Multifluorescence live analysis of herpes simplex virus type-1 replication

Abstract

Keywords

ASJC Scopus subject areas

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