TY - JOUR
T1 - Multidirectional approach to study peritoneal dialysis fluid biocompatibility in a chronic peritoneal dialysis model in the rat
AU - Wieczorowska-Tobis, Katarzyna
AU - Polubinska, Alicja
AU - Wisniewska, Justyna
AU - Pawlaczyk, K.
AU - Kuzlan-Pawlaczyk, M.
AU - Filas, Violeta
AU - Breborowicz, Andrzej
AU - Oreopoulos, Dimitrios G.
PY - 2001
Y1 - 2001
N2 - Background. Peritoneal dialysis causes the functional and morphological changes in the peritoneum that result from the bioincompatibility of dialysis solutions. We present a model of chronic peritoneal dialysis in the rat that can be used for testing the biocompatibility of dialysis fluids. Methods and Results. Long-term exposure of the peritoneum to dialysis solutions can be performed in rats with implanted peritoneal catheters. Sampling of the dialysate allows the evaluation of intraperitoneal inflammation by examining cell differential and dialysate cytokine levels. Peritoneal permeability can be evaluated at designed time intervals with the peritoneal equilibration test (PET). At the end of dialysis, peritoneal histology is studied with light and electron microscopy. Conclusions. Such a multidirectional approach is an effective way to test biocompatibility of dialysis solutions.
AB - Background. Peritoneal dialysis causes the functional and morphological changes in the peritoneum that result from the bioincompatibility of dialysis solutions. We present a model of chronic peritoneal dialysis in the rat that can be used for testing the biocompatibility of dialysis fluids. Methods and Results. Long-term exposure of the peritoneum to dialysis solutions can be performed in rats with implanted peritoneal catheters. Sampling of the dialysate allows the evaluation of intraperitoneal inflammation by examining cell differential and dialysate cytokine levels. Peritoneal permeability can be evaluated at designed time intervals with the peritoneal equilibration test (PET). At the end of dialysis, peritoneal histology is studied with light and electron microscopy. Conclusions. Such a multidirectional approach is an effective way to test biocompatibility of dialysis solutions.
KW - Inflammatory reaction
KW - Morphology
KW - Peritoneal dialysis
KW - Peritoneal transport
KW - Rat model
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U2 - 10.1093/ndt/16.3.655
DO - 10.1093/ndt/16.3.655
M3 - Article
C2 - 11239060
AN - SCOPUS:0035097029
SN - 0931-0509
VL - 16
SP - 655
EP - 656
JO - Nephrology Dialysis Transplantation
JF - Nephrology Dialysis Transplantation
IS - 3
ER -