Multi-parameter fluorescence-activated cell sorting and analysis of stem and progenitor cells in myeloid malignancies

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20 Citations (Scopus)

Abstract

Owing to the discovery that rare leukemia-initiating cells (or leukemia stem cells, LSC) give origin to and propagate a hierarchical cellular organization of variably differentiated leukemic blasts, the analysis of precisely defined stem and progenitor cells have increasingly gained importance. Emergence of multi-parameter high-speed fluorescence-activated cell sorting (FACS) for the subfractionation of hematopoietic progenitor cells has allowed research on the biology of the cell-of-origin for LSCs and of LSCs as potential (or essential) therapeutic targets that may escape chemotherapy and consequently contribute to disease relapse. This review introduces the current state-of-the-art methods for the fractionation of hematopoietic stem and progenitor cells, with particular focus on myeloid malignancies. As many aspects of human normal and malignant hematopoiesis are frequently modeled in animal studies, we also provide an overview of hematopoietic stem and progenitor cell purification methods that are commonly utilized for research in murine models of disease.

Original languageEnglish (US)
Pages (from-to)391-401
Number of pages11
JournalBest Practice and Research: Clinical Haematology
Volume23
Issue number3
DOIs
StatePublished - Sep 2010

Fingerprint

Hematopoietic Stem Cells
Sorting
Flow Cytometry
Stem Cells
Fluorescence
Cells
Chemotherapy
Fractionation
Stem cells
Purification
Neoplasms
Animals
Leukemia
Hematopoiesis
Research
Cell Biology
Recurrence
Drug Therapy
Therapeutics

Keywords

  • Acute myeloid leukemia
  • Cancer stem cell
  • FACS
  • Hematopoietic stem cell
  • Leukemia
  • Leukemia stem cell
  • Leukemia-initiating
  • Myelodysplastic syndrome
  • Myeloid
  • Progenitor cell

ASJC Scopus subject areas

  • Oncology
  • Clinical Biochemistry

Cite this

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abstract = "Owing to the discovery that rare leukemia-initiating cells (or leukemia stem cells, LSC) give origin to and propagate a hierarchical cellular organization of variably differentiated leukemic blasts, the analysis of precisely defined stem and progenitor cells have increasingly gained importance. Emergence of multi-parameter high-speed fluorescence-activated cell sorting (FACS) for the subfractionation of hematopoietic progenitor cells has allowed research on the biology of the cell-of-origin for LSCs and of LSCs as potential (or essential) therapeutic targets that may escape chemotherapy and consequently contribute to disease relapse. This review introduces the current state-of-the-art methods for the fractionation of hematopoietic stem and progenitor cells, with particular focus on myeloid malignancies. As many aspects of human normal and malignant hematopoiesis are frequently modeled in animal studies, we also provide an overview of hematopoietic stem and progenitor cell purification methods that are commonly utilized for research in murine models of disease.",
keywords = "Acute myeloid leukemia, Cancer stem cell, FACS, Hematopoietic stem cell, Leukemia, Leukemia stem cell, Leukemia-initiating, Myelodysplastic syndrome, Myeloid, Progenitor cell",
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KW - Cancer stem cell

KW - FACS

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KW - Leukemia

KW - Leukemia stem cell

KW - Leukemia-initiating

KW - Myelodysplastic syndrome

KW - Myeloid

KW - Progenitor cell

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